Protein Info for GFF1126 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Isoleucyl-tRNA synthetase (EC 6.1.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 956 TIGR00392: isoleucine--tRNA ligase" amino acids 27 to 854 (828 residues), 1017.1 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 40 to 654 (615 residues), 530.5 bits, see alignment E=8.3e-163 PF09334: tRNA-synt_1g" amino acids 70 to 213 (144 residues), 38.8 bits, see alignment E=1.5e-13 PF01406: tRNA-synt_1e" amino acids 590 to 662 (73 residues), 27.3 bits, see alignment 7e-10 PF08264: Anticodon_1" amino acids 699 to 854 (156 residues), 94.6 bits, see alignment E=1.6e-30 PF06827: zf-FPG_IleRS" amino acids 916 to 945 (30 residues), 39.6 bits, see alignment (E = 1e-13)

Best Hits

Swiss-Prot: 91% identical to SYI_CITK8: Isoleucine--tRNA ligase (ileS) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 73% identity to asa:ASA_0684)

MetaCyc: 91% identical to isoleucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Isoleucine--tRNA ligase. [EC: 6.1.1.5]; 6.1.1.5 [EC: 6.1.1.5]; 3.1.1.- [EC: 6.1.1.5]; RXN-23924 [EC: 6.1.1.5]

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (956 amino acids)

>GFF1126 Isoleucyl-tRNA synthetase (EC 6.1.1.5) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
VFIKPKYGTENLMSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAAKKGK
KTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGFDSPYVPGWDCHGLPIELKVEQ
EFGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFKTEANIIRA
LGRIIKNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFRAVDQDAVKAKFGL
PGVSGPVSLVIWTTTPWTLPANRAISLAPDFDYALVQIDGQAVILAKDLVESVMQRIGAA
EYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHTAPGHGPDDYVIGQK
YGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIELLKEKGALLHVEKMQHSYPCCWR
HKTPIIFRATPQWFVSMDKEGLRQQSLKEIKGVQWIPDWGQARIESMVANRPDWCISRQR
TWGVPMSLFVHKETQELLPIERTLAAMEEVAKRVEVDGIQAWWDLDPKEILGEDADQYEK
VPDTLDVWFDSGSTSYSVVDARPEFAGHAADMYLEGSDQHRGWFMSSLMISVAMKGKAPY
RQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADILRLWVASTDYTGEMAVSDEILK
RAADSYRRIRNTARFLLANLNGFNPATDMVKPEEMVVLDRWAVGCAKTAQQEILKAYEAY
DFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKADSVARRSCQTALYHIAEALVRWMAPI
MSFTADEIWGYLPGEREKYVFTGEWYDGLFGLEENEEFNDAFWDDVRYIKDQVNKELENQ
KANGIKSNLEAKVTLKYADDANGTIKKLKLLGEEVRFIFITSQFVISEQAGGIDDENIQY
NAGNTTVQAVVTRAEGDKCPRCWHYTTDVGKVAEHADICGRCVSNIAGNGEQRKFA