Protein Info for GFF1123 in Variovorax sp. SCN45

Annotation: GTP 3',8-cyclase (EC 4.1.99.22)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 385 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR02666: molybdenum cofactor biosynthesis protein A" amino acids 37 to 385 (349 residues), 379.5 bits, see alignment E=6.3e-118 PF04055: Radical_SAM" amino acids 50 to 220 (171 residues), 108 bits, see alignment E=8.8e-35 PF13353: Fer4_12" amino acids 55 to 146 (92 residues), 28.1 bits, see alignment E=3.5e-10 PF06463: Mob_synth_C" amino acids 230 to 356 (127 residues), 132.9 bits, see alignment E=9.9e-43

Best Hits

Swiss-Prot: 66% identical to MOAA_RALSO: GTP 3',8-cyclase (moaA) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K03639, molybdenum cofactor biosynthesis protein (inferred from 90% identity to vap:Vapar_2343)

Predicted SEED Role

"Molybdenum cofactor biosynthesis protein MoaA" in subsystem Molybdenum cofactor biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (385 amino acids)

>GFF1123 GTP 3',8-cyclase (EC 4.1.99.22) (Variovorax sp. SCN45)
MTLMKHSSSTVIPISAVGRARPAVAVPEVAVPATGQLLDRLGRPMTDLRISVTDRCNFRC
SYCMPKDVFDKDYQYLPHGSLLSFEEMTRLARLFATHGVRKIRLTGGEPLLRKNIEGLIS
QLAEIRTPAGELLALTLTTNGSLLRRKASALRAAGLQRVTVSLDSLDDAVFRHMNDVDFP
VADVLAGIDAALAAGLGPIKVNMVVKRGTNEQEILPMARYFREQHGGKVVLRFIEYMDVG
ATNGWRMDEVLPSAEVIARIHAEFPLVPLQASTPGETAQRWAYADGSGEIGVISSVTQAF
CHDCSRARLSTEGKLYLCLFASAGHDLRPLLRGTASDEDIASAIGHIWQGRADRYSELRA
LRTTDAEGDAAGQTPRRVEMSYIGG