Protein Info for PS417_05665 in Pseudomonas simiae WCS417

Annotation: methionine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 683 PF09334: tRNA-synt_1g" amino acids 7 to 395 (389 residues), 525.9 bits, see alignment E=1.4e-161 TIGR00398: methionine--tRNA ligase" amino acids 7 to 535 (529 residues), 583.2 bits, see alignment E=5.2e-179 PF00133: tRNA-synt_1" amino acids 11 to 234 (224 residues), 33.3 bits, see alignment E=6.2e-12 PF19303: Anticodon_3" amino acids 426 to 517 (92 residues), 27.1 bits, see alignment E=9.1e-10 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 566 to 683 (118 residues), 137.5 bits, see alignment E=2.7e-44 PF01588: tRNA_bind" amino acids 587 to 681 (95 residues), 84.7 bits, see alignment E=1e-27

Best Hits

Swiss-Prot: 98% identical to SYM_PSEFS: Methionine--tRNA ligase (metG) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 98% identity to pfs:PFLU1161)

MetaCyc: 65% identical to methionine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Methionine--tRNA ligase. [EC: 6.1.1.10]; 6.1.1.10 [EC: 6.1.1.10]; RXN-23924 [EC: 6.1.1.10]

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TYK7 at UniProt or InterPro

Protein Sequence (683 amino acids)

>PS417_05665 methionine--tRNA ligase (Pseudomonas simiae WCS417)
MSEPRKILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKHRGNQCIYVCADDAHGSAIML
RAEKEGITPEQLIANVQAEHSADFAEFLVDFDNFHSTHSEENRELSSQIYLRLKEAGHID
QRSVTQYFDPEKKMFLADRFIKGTCPKCGTEDQYGDNCEKCGATYAPTELKNPKSAISGA
TPVLKDSQHFFFKLPDFQQMLQTWTRSGTLQDAVANKIAEWLDAGLQQWDISRDAPYFGF
EIPGEPGKYFYVWLDAPIGYMASFKNLCDRTPELDFDAFWNKDSTAELYHFIGKDIVNFH
ALFWPAMLEGSGYRKPTGIAVHGYLTVNGQKMSKSRGTFIKARTYLDHLSPEYLRYYYAS
KLGRGVDDLDLNLEDFVQKVNSDLVGKVVNIASRCAGFIHKGNAGLLVAENAAPELTDAF
LAAAPSIADAYEARDFARAMREIMGLADRANAWIADKAPWSLNKQEGKQAEVQAICATGV
NLFRQLVIFLKPVLPLLAADAEAFLNVAPLTWNDHATLLSNHQLNEFKPLMTRIDPVKVQ
AMTDASKEDLVASQTDTGSAAPVGNGELAKDPISAEIDFDAFAAVDLRVALIVKAEAVEG
ADKLLRLTLDLGGEQRNVFSGIKSAYPDPSKLDGRLTMMIANLKPRKMKFGVSEGMVMAA
GPGGEEIYLLSPDSGAKPGQRIK