Protein Info for GFF1105 in Xanthobacter sp. DMC5

Annotation: Bifunctional protein HldE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 487 TIGR02198: bifunctional protein RfaE, domain I" amino acids 4 to 315 (312 residues), 371.6 bits, see alignment E=4.7e-115 PF00294: PfkB" amino acids 12 to 307 (296 residues), 132.3 bits, see alignment E=2.5e-42 TIGR02199: bifunctional protein RfaE, domain II" amino acids 342 to 482 (141 residues), 186.7 bits, see alignment E=3e-59 TIGR00125: cytidyltransferase-like domain" amino acids 352 to 417 (66 residues), 49.3 bits, see alignment E=6.6e-17 PF01467: CTP_transf_like" amino acids 354 to 446 (93 residues), 63.6 bits, see alignment E=2.3e-21

Best Hits

Swiss-Prot: 53% identical to HLDE_GLUDA: Bifunctional protein HldE (hldE) from Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5)

KEGG orthology group: K03272, D-beta-D-heptose 7-phosphate kinase / D-beta-D-heptose 1-phosphate adenosyltransferase [EC: 2.7.1.- 2.7.7.-] (inferred from 58% identity to bid:Bind_1031)

Predicted SEED Role

"ADP-heptose synthase (EC 2.7.-.-) / D-glycero-beta-D-manno-heptose 7-phosphate kinase" in subsystem LOS core oligosaccharide biosynthesis (EC 2.7.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.-.-, 2.7.1.-, 2.7.7.-

Use Curated BLAST to search for 2.7.-.- or 2.7.1.- or 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (487 amino acids)

>GFF1105 Bifunctional protein HldE (Xanthobacter sp. DMC5)
MSVIDQFARVRVLCIGDVMLDRFVMGAARRISPESPVPVLSVTGSNTIAGGSANVARNIA
SLGGKCTLVGVIGTDACGAELKRILDETPGVASAFVEVAQRPTTEKIRFVAQGQHMLRSD
SEDSSPVAPDVEEAIVARIAAVLPEHDVVVLSDYAKGVLTTGVIRRVVELATRRGAPVIV
DPKSSDLARYDGASLVTPNLHETYLATGIQGDDDASAVAAARKVLAGTTIGAVLVTRSEK
GMTLVQRTADPVHIPTAAREVADVVGAGDTVIAALSLVVGAGGDLQEAAYIANAAAGVVV
AKRGTATVSRDELLVELNRQSPTDGNPSSVKVVSRADAAAQVAAWQQHGLAVGFTNGCFD
IVHVGHVGIIEFSRAHCDRLVVAVNSDASVKRLKGPTRPVNGEIDRAQVIAALGAVDLVV
VFDEDTPKELIEELQPDVLVKGADYQVSEIVGADSVLARGGKVLRFELVPGRSTTKVIGR
MTEGVSA