Protein Info for PS417_05580 in Pseudomonas simiae WCS417

Annotation: glycerol uptake facilitator GlpF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 285 transmembrane" amino acids 18 to 40 (23 residues), see Phobius details amino acids 42 to 45 (4 residues), see Phobius details amino acids 49 to 82 (34 residues), see Phobius details amino acids 91 to 111 (21 residues), see Phobius details amino acids 148 to 169 (22 residues), see Phobius details amino acids 181 to 204 (24 residues), see Phobius details amino acids 233 to 254 (22 residues), see Phobius details PF00230: MIP" amino acids 6 to 254 (249 residues), 210.3 bits, see alignment E=1.7e-66 TIGR00861: MIP family channel proteins" amino acids 16 to 254 (239 residues), 233.4 bits, see alignment E=1.4e-73

Best Hits

Swiss-Prot: 83% identical to GLPF_PSEAE: Glycerol uptake facilitator protein (glpF) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02440, glycerol uptake facilitator protein (inferred from 99% identity to pfs:PFLU1143)

MetaCyc: 71% identical to glycerol facilitator (Escherichia coli K-12 substr. MG1655)
RXN0-7189; RXN0-7191; TRANS-RXN-131; TRANS-RXN0-460; TRANS-RXN0-536; TRANS-RXN0-537; TRANS-RXN0-551

Predicted SEED Role

"Glycerol uptake facilitator protein" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerol fermenation to 1,3-propanediol

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TV87 at UniProt or InterPro

Protein Sequence (285 amino acids)

>PS417_05580 glycerol uptake facilitator GlpF (Pseudomonas simiae WCS417)
MTTALQQPSLSSQCMAEFLGTALLIFFGTGCVAALKVAGASFGLWEISIIWGVGVSMAIY
LSAGISGAHLNPAVSIALCIFADFDKRKLPFYILAQIAGAFCSAALVYTLYSNLFFDYEQ
THHMVRGSQASLELASVFSTYPHALLSTAQAFLVEMVITAILMGVIMALTDDNNGLPRGP
LAPLLIGLLIAVIGSAMGPLTGFAMNPARDFGPKLMTFFAGWGEMAFTGGRDIPYFLVPI
FAPIVGACLGAAAYRGLIARHLPSAAPAIDEETPDTAVNGKTRIS