Protein Info for PS417_05565 in Pseudomonas simiae WCS417

Annotation: glycerol-3-phosphate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01266: DAO
  • amino acids 16 to 369 (354 residues), 205.2 bits, E=3.4e-64
• PF16901: DAO_C
  • amino acids 390 to 500 (111 residues), 50.2 bits, E=3.6e-17

Best Hits

Swiss-Prot: 73% identical to GLPD_PSEAE: Glycerol-3-phosphate dehydrogenase (glpD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00111, glycerol-3-phosphate dehydrogenase [EC: 1.1.5.3] (inferred from 97% identity to pfs:PFLU1140)

MetaCyc: 74% identical to aerobic glycerol-3-phosphate dehydrogenase (Pseudomonas putida KT2440)
Glycerol-3-phosphate dehydrogenase. [EC: 1.1.5.3]; 1.1.5.3 [EC: 1.1.5.3]; 1.1.5.3 [EC: 1.1.5.3]

Predicted SEED Role

"Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Respiratory dehydrogenases 1 (EC 1.1.5.3)

MetaCyc Pathways

• glycerol and glycerophosphodiester degradation (4/4 steps found)
• glycerol degradation I (3/3 steps found)
• glycerol-3-phosphate shuttle (2/2 steps found)
• glycerol-3-phosphate to cytochrome bo oxidase electron transfer (2/2 steps found)
• glycerophosphodiester degradation (2/2 steps found)
• nitrate reduction IX (dissimilatory) (2/2 steps found)
• glycerol 3-phosphate to cytochrome aa₃ oxidase electron transfer (1/2 steps found)
• glycerol-3-phosphate to fumarate electron transfer (1/2 steps found)
• glycerol-3-phosphate to hydrogen peroxide electron transport (1/2 steps found)
• sn-glycerol 3-phosphate anaerobic respiration (1/3 steps found)

KEGG Metabolic Maps

• Glycerophospholipid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.3

Sequence Analysis Tools

See A0A1N7TYI8 at UniProt or InterPro

Protein Sequence (512 amino acids)

>PS417_05565 glycerol-3-phosphate dehydrogenase (Pseudomonas simiae WCS417)
MNPSTLPAPPLAEVYDVAVIGGGINGVGIAADAAGRGLSVFLCEKDDLASHTSSASSKLI
HGGLRYLEHYEFRLVREALAEREVLLAKAPHIVKQMRFVLPHRPHLRPAWMIRAGLFLYD
NLGKREKLEGSKSLKFGPDSPLKSEITKGFEYSDCWVDDARLVVLNAMAAREKGAHIHTQ
TRCISAHRSNGMWEMNMERADGSLFSIRARALVNAAGPWVAKFIKDDLKLDSPYGIRLIQ
GSHLIVPKLYEGAHAHILQNEDQRIVFTIPYLNHLTIIGTTDREYTGDPAKVAITEGETD
YMLKVVNAHFKKQLSRDDIVHTYSGVRPLCNDESDNPSAITRDYTLALSGGTGEAPILSV
FGGKLTTYRKLAESAMAQLAPYFTQMRPSWTAKASLPGGEDMTTPEALAEAIRSKFDWVP
SEIARRWSTTYGSRTWRLLEGVQSLADLGEHLGGGLYTREVDYLCAEEWATQPQDVLWRR
TKLGLFTTPAEQANVQRYLSTVEQNRSKIEAA