Protein Info for GFF109 in Sphingobium sp. HT1-2

Annotation: 5-aminolevulinate synthase (EC 2.3.1.37)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01821: 5-aminolevulinic acid synthase
  • amino acids 2 to 402 (401 residues), 663 bits, E=7.6e-204
• PF00155: Aminotran_1_2
  • amino acids 48 to 391 (344 residues), 247.6 bits, E=1.2e-77

Best Hits

Swiss-Prot: 61% identical to HEM1_BRADU: 5-aminolevulinate synthase (hemA) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K00643, 5-aminolevulinate synthase [EC: 2.3.1.37] (inferred from 95% identity to sch:Sphch_0777)

MetaCyc: 57% identical to 5-aminolevulinate synthase 2 (Cereibacter sphaeroides)
5-aminolevulinate synthase. [EC: 2.3.1.37]

Predicted SEED Role

"5-aminolevulinate synthase (EC 2.3.1.37)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 2.3.1.37)

MetaCyc Pathways

• superpathway of heme b biosynthesis from glycine (7/8 steps found)
• tetrapyrrole biosynthesis II (from glycine) (4/4 steps found)
• 2-amino-3-hydroxycyclopent-2-enone biosynthesis (2/4 steps found)
• superpathway of bacteriochlorophyll a biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.37

Protein Sequence (408 amino acids)

>GFF109 5-aminolevulinate synthase (EC 2.3.1.37) (Sphingobium sp. HT1-2)
VNYKHIFSQAIDRLHSEGRYRVFIDILRNKGAFPNARCFHGHNGPKPITVWCSNDYLAMG
QHPKVVAAMEEALHDVGAGSGGTRNIGGNTHYHVDLEAELADLHGKEAALLFTSGYVSNE
ATLSTLAKILPGCIIFSDELNHASMIAGIRNSGCEKRVFRHNDLDHLRELLAAEDPEAPK
LIAFESVYSMDGDVAPIAEICDLADEFNALTYLDEVHAVGMYGARGGGISERDDVAHRLT
IIEGTLGKAFGVMGGYIAADQMIVDVIRSYAPGFIFTTSLSPVLVAGVLASVRHLKGSSA
EREGQQASAAKLKQLMRDAGLPVMNSVTHIVPLMVGDPVKAKRISDILLAEYGAYVQPIN
YPTVPRGTERLRFTPGPAHNEEMMRDLVSALVEIWDRLELELLERQAA