Protein Info for GFF109 in Pseudomonas sp. DMC3

Annotation: Low specificity L-threonine aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 88% identical to LTAE_PSEUN: Low specificity L-threonine aldolase (ltaE) from Pseudomonas sp. (strain NCIMB 10558)

KEGG orthology group: K01620, threonine aldolase [EC: 4.1.2.5] (inferred from 98% identity to pfo:Pfl01_5210)

Predicted SEED Role

"Low-specificity L-threonine aldolase (EC 4.1.2.48)" (EC 4.1.2.48)

MetaCyc Pathways

glycine biosynthesis IV (1/1 steps found)
L-threonine degradation IV (1/2 steps found)
superpathway of L-threonine metabolism (11/18 steps found)

KEGG Metabolic Maps

Glycine, serine and threonine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.2.48, 4.1.2.5

Use Curated BLAST to search for 4.1.2.48 or 4.1.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (346 amino acids)

>GFF109 Low specificity L-threonine aldolase (Pseudomonas sp. DMC3)
MTDKSQQFASDNYSGICPEAWAAMEQANHGHQRAYGDDEWTARAADHFRKLFETDCEVFF
AFNGTAANSLALSSLCQSYHSVICSETAHVETDECGAPEFFSNGSKLLIAGTENGKITPQ
SIREVALKRQDIHYPKPRVVTLTQATEVGSVYTPEEVRAISATCKELGLHLHMDGARFSN
ACAFLGCSPADLTWKAGVDVLCFGGTKNGMAVGEAILFFNHKLAEDFDYRCKQAGQLASK
MRFLSAPWVGILENDAWLKYARHANHCAQLLAELVSDIPGVELMFPVQANGVFLQLSEPA
IAALTAKNWRFYTFIGKGGARFMCSWDTEEERVRELARDIREVMSA