Protein Info for GFF1086 in Pseudomonas sp. DMC3

Annotation: Uric acid permease PucK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 transmembrane" amino acids 21 to 44 (24 residues), see Phobius details amino acids 50 to 71 (22 residues), see Phobius details amino acids 80 to 97 (18 residues), see Phobius details amino acids 103 to 124 (22 residues), see Phobius details amino acids 134 to 156 (23 residues), see Phobius details amino acids 169 to 186 (18 residues), see Phobius details amino acids 195 to 216 (22 residues), see Phobius details amino acids 238 to 258 (21 residues), see Phobius details amino acids 318 to 341 (24 residues), see Phobius details amino acids 346 to 365 (20 residues), see Phobius details amino acids 377 to 398 (22 residues), see Phobius details amino acids 405 to 427 (23 residues), see Phobius details TIGR00801: uracil-xanthine permease" amino acids 14 to 425 (412 residues), 364.4 bits, see alignment E=8.1e-113 PF00860: Xan_ur_permease" amino acids 16 to 395 (380 residues), 337.7 bits, see alignment E=3.9e-105 TIGR03173: xanthine permease" amino acids 21 to 428 (408 residues), 496.8 bits, see alignment E=4.3e-153

Best Hits

Swiss-Prot: 43% identical to PBUX_BACSU: Xanthine permease (pbuX) from Bacillus subtilis (strain 168)

KEGG orthology group: K03458, nucleobase:cation symporter-2, NCS2 family (inferred from 94% identity to pfo:Pfl01_2729)

MetaCyc: 42% identical to urate:H+ symporter (Escherichia coli K-12 substr. MG1655)
RXN-5076; TRANS-RXN0-530

Predicted SEED Role

"Xanthine-uracil permease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (446 amino acids)

>GFF1086 Uric acid permease PucK (Pseudomonas sp. DMC3)
MTTQRVHPVDEVLPLRQLVTFGLQHVLVMYAGAVAVPLILGSAMGLTSAQVVLLINANLL
TSGVATLIQTLGFWKFGARLPLIQGCSFIALAPMIMIGKEFGLSQIFGAVIAAGFLTIAL
APVFSRLLRFFPPVVIGSLITIIGISLMPAAAIWLGGGNPDSPDFGRPANLLLGLATVSV
TLVIYAKFSGFIGNLSVLIGLFVGSLIAAACGMTHFNRVSEAAWFELSAPMAFGPPEFSL
VPILIMTLAMLVIMAETTGNCLAIGKLVGKPTTQQTLGNAFRADGLSTMLGGVFNSFPYN
AFTQNTGLIALSSIKSRFVVAAAGAIMVLMGLFPKLGALIAAVPTPVLGGCAIVMFGMTT
VAGIQELSRVQFEGTRNAIIVAVSVSVGVLPMSFPALFEHVGPTLKLVLDSGIFLGAITA
ILLNVLLNKQKPSTDAVGASTAELAD