Protein Info for GFF108 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Tryptophanyl-tRNA synthetase (EC 6.1.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 TIGR00233: tryptophan--tRNA ligase" amino acids 2 to 331 (330 residues), 299.2 bits, see alignment E=2.1e-93 PF00579: tRNA-synt_1b" amino acids 4 to 282 (279 residues), 182.7 bits, see alignment E=5.1e-58

Best Hits

Swiss-Prot: 72% identical to SYW_XANCP: Tryptophan--tRNA ligase (trpS) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K01867, tryptophanyl-tRNA synthetase [EC: 6.1.1.2] (inferred from 76% identity to pol:Bpro_1001)

Predicted SEED Role

"Tryptophanyl-tRNA synthetase (EC 6.1.1.2)" (EC 6.1.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (430 amino acids)

>GFF108 Tryptophanyl-tRNA synthetase (EC 6.1.1.2) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKTQRVLTGITTSGTPHLGNYVGAIRPTVRASRLAEVESFYFLADYHALIKVGEPARVQR
STLEIAATWLACGLDPEKVTFYRQSDIPEIPELTWLLTCVTGKGILNRAHAYKASVDKNN
AAGEDPDAGVTAGLFMYPVLMAADIVMFNAHKVPVGRDQIQHIEMARDMASSFNHLYGEH
FTLPEAVTEDHVALLPGLDGRKMSKSYDNTIPLFVPREQLRKLIMGIVTDSRAPGEPKST
EGSALFQLYQAFASAEETTALAKAFADGIAWGDAKQTLFERLDREIAPMREVYDQLIQDP
ARIEKTLKAGAEKARAIATPFTARLREAVGLRRLDSVVSASAAKAAKSAAPAFKQYRESD
GLFYFKLVDAGGQLLLQSTGFASGKEAAGAIARLQQQGAQALEGLQGQLTTQADPAALAQ
ALAHFGPRDE