Protein Info for GFF1056 in Methylophilus sp. DMC18

Annotation: Bifunctional uridylyltransferase/uridylyl-removing enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 858 TIGR01693: protein-P-II uridylyltransferase" amino acids 30 to 857 (828 residues), 815.3 bits, see alignment E=3.5e-249 PF01909: NTP_transf_2" amino acids 60 to 97 (38 residues), 28.7 bits, see alignment (E = 2.2e-10) PF08335: GlnD_UR_UTase" amino acids 164 to 297 (134 residues), 148.6 bits, see alignment E=1.9e-47 PF01966: HD" amino acids 439 to 538 (100 residues), 39.5 bits, see alignment E=9.4e-14

Best Hits

KEGG orthology group: K00990, [protein-PII] uridylyltransferase [EC: 2.7.7.59] (inferred from 56% identity to mep:MPQ_1486)

Predicted SEED Role

"[Protein-PII] uridylyltransferase (EC 2.7.7.59)" in subsystem Ammonia assimilation (EC 2.7.7.59)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.59

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (858 amino acids)

>GFF1056 Bifunctional uridylyltransferase/uridylyl-removing enzyme (Methylophilus sp. DMC18)
MTVIGKEHLKHWRASVQLTRQQLADAYLAKPQAKRLLQQHTRSLDLLLKQIWQAYALPDS
ACLVAVGGYGRGDMFPYSDIDLLILMPENSDAALQTLVESLIGTLWDIGLNIGHSVRSLK
ECLDEAQLDVTIMTNLLEARWLSGDKKTFQSLTQLLHAQLDQARFFAEKLREQQIRHAKF
HDTAYNLEPNVKESPGGLRDLHMVLWVAQSQGLGSDWNALAQAGLLSRHEAIQLKKHERL
LQNLRIRLHLMAKRREDRLVFDFQNTLAEQMGYETNPQKRASEQLMQGFYRSAKAVIIEN
EVLLKLLHARIQPETKPPQALTEDFSLSQGLLSANAPDLFQRKPETILQCFQLLQQYPEI
TGFSAPLVRALQAAQPLINHQFRQRANHKALFLQILHSREGVHRSLRRMNRYGILGRYIP
VFGRVIAQMQHDLFHVYTVDEHTLNVLRNLRRFAKPDLRHEFPLCSQLFNAFSQPHLLYL
AAIFHDIAKGRGGDHSLLGTLDARRFCKQHGLSKTDTELVAWLVEAHLKLSSTAQKSDLS
DPDVIEAFAHLVGTEYRLTALYLLTVADIRGTSPNVWNAWKAKLLESLFLQTRRVLQQSL
NTEAQLSLRKQEVLQKLSSFNLKESSVAALWQAFGNGYFSRFDSDEIAWQSRLLIPHLNT
EQAIVRARLSPKGDGIQVMIYSRDQKEIFARICHFFDSMQYNIVQAKIYTTAHGYALDNF
IVLEPETRQISYNGLLKHIEQGLNTQLISHEAIPAPIRGRVSRQVKHMPISTQVSFKAVP
NPQDADQPFQQLDIIANDRPGLLASMALIFQQHGIALHNAKINTLGNRVEDSFLISACNN
GLLDATSITNLTQALHQI