Protein Info for GFF1036 in Variovorax sp. SCN45

Annotation: diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 655 transmembrane" amino acids 16 to 36 (21 residues), see Phobius details amino acids 48 to 70 (23 residues), see Phobius details amino acids 82 to 105 (24 residues), see Phobius details amino acids 117 to 136 (20 residues), see Phobius details amino acids 152 to 173 (22 residues), see Phobius details amino acids 183 to 203 (21 residues), see Phobius details PF00990: GGDEF" amino acids 222 to 379 (158 residues), 40.4 bits, see alignment E=2.6e-14 PF00563: EAL" amino acids 412 to 631 (220 residues), 176.7 bits, see alignment E=5.2e-56

Best Hits

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (655 amino acids)

>GFF1036 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) (Variovorax sp. SCN45)
VQEFFIAAWRAEFANYPLLILQAAALLGILSIALLLSRTPTERFESRTLLYGCIFGATSF
SLIALASQFFNLPSKPYLSSDLLFLGGLLGGWKGGAITLAMTILGRLIFGGVNQVEAFLL
DMSITAAGGVAAHLIYRKKAIKDFGWSDVLKIWGTRMACSLFSSAIIFALELVPPKISIH
VTTLQIFGFSTSLFILGSVIALLRSDALVRESYFQRMQMYRTDLLSGLPNRRALSEYLEI
LLKRDQTQHVLLTFEVGNLKEMVRTLGHDWTDKFWGHLARELMRQESLKLTSNGKSKCFQ
LSDLALAFVVKETSVKQIENSQLMPRLYADITEHLQVAGGPYSSAQLRCGITNVRMAGET
NSSSVLRNISLALQSSNQQIRYFHDSFSAQADRDADVHELMIGWIRTVNPPMSYQPKFDL
ETRQICGAEALLRANSAEGISLSPLYVLDVATRYQLLLKLEWCIVEMVIREIANCMASGF
HMPLSVNISAASITVPGLGERILDRLSRYSVPFELLSIEITESGHVPDMDTVRENVHRLH
SAGVGLSLDDFGAGYSTLTTLAKIPFNEVKIDYAMVSMIEQPRMNKAIGLALESATRYGA
TLVAEGVETEAQLAILIDMGIKFGQGFLLSKAVPMDDLIELARRERGERLYGDLE