Protein Info for GFF1026 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Ferredoxin reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 391 PF07992: Pyr_redox_2" amino acids 5 to 303 (299 residues), 216 bits, see alignment E=1.9e-67 PF00070: Pyr_redox" amino acids 146 to 224 (79 residues), 54.8 bits, see alignment E=2.7e-18 PF14759: Reductase_C" amino acids 322 to 385 (64 residues), 62.3 bits, see alignment E=1.3e-20

Best Hits

Swiss-Prot: 41% identical to CNDC1_SPHSD: Chloroacetanilide N-alkylformylase, ferredoxin reductase component (cndC1) from Sphingomonas wittichii (strain DC-6 / KACC 16600)

KEGG orthology group: K00529, ferredoxin--NAD+ reductase [EC: 1.18.1.3] (inferred from 57% identity to mno:Mnod_0328)

MetaCyc: 40% identical to rubredoxin-NAD(+) reductase (Pseudomonas oleovorans)
Rubredoxin--NAD(+) reductase. [EC: 1.18.1.1]; Alkane 1-monooxygenase. [EC: 1.18.1.1, 1.14.15.3]

Predicted SEED Role

"Ferredoxin reductase" in subsystem Anaerobic respiratory reductases

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.1.1, 1.18.1.3

Use Curated BLAST to search for 1.14.15.3 or 1.18.1.1 or 1.18.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (391 amino acids)

>GFF1026 Ferredoxin reductase (Hydrogenophaga sp. GW460-11-11-14-LB1)
MAHGTVIVGTGQGGFQLAASLRDSGYADPITLVGDEPGLPYQRPPLSKAFLQGKVDDTGL
LLRPPPFYQRIDARVLGGTRVARIDRAARGVHLSSGQTLPYDHLVLATGATARTLCVPGA
GLSGVHVLRGLEDARRIREQLARARSVAVVGAGFIGLELAAVARGQGLPMHVIEATARPL
LRALSEPMARHLQAFHEAQGTQFHLNTQLDAIEGEDGRATGLRLHGGGRIVADLIVIAVG
VTPNDQLARDAGLAVGNGIEVDELLLTSDAAISALGDCANFPFAADGGSRVRIESVQNAV
DQARCIAARLCGQPLPYAKVPWFWSEQGPCRLQMAGLAAPGDRSDIEGDPASGRFSVLRY
RGDQLSAVESLNDPAAYMRARTALAPVLAPT