Protein Info for GFF1024 in Methylophilus sp. DMC18

Annotation: Formate--tetrahydrofolate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01268: FTHFS
  • amino acids 3 to 547 (545 residues), 812 bits, E=1.1e-248

Best Hits

Swiss-Prot: 61% identical to FTHS_METS4: Formate--tetrahydrofolate ligase (fhs) from Methylobacterium sp. (strain 4-46)

KEGG orthology group: K01938, formate--tetrahydrofolate ligase [EC: 6.3.4.3] (inferred from 66% identity to meh:M301_1217)

Predicted SEED Role

"Formate--tetrahydrofolate ligase (EC 6.3.4.3)" in subsystem Folate Biosynthesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 6.3.4.3)

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• folate polyglutamylation (5/5 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• formaldehyde oxidation VII (THF pathway) (3/4 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (2/3 steps found)
• folate transformations II (plants) (7/11 steps found)
• folate transformations III (E. coli) (5/9 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (4/9 steps found)
• L-histidine degradation III (1/6 steps found)
• folate transformations I (6/13 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (2/10 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)
• purine nucleobases degradation II (anaerobic) (8/24 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.3

Protein Sequence (547 amino acids)

>GFF1024 Formate--tetrahydrofolate ligase (Methylophilus sp. DMC18)
MLSDIAIAQAATLTPIVNIASKLGLVADDVHAYGQHMAKLTQATVNRLQAQPAKAKLILV
TAINPTPAGEGKTTTTIGLTDALNQAGYQAMACLREPSLGPVFGMKGGATGGGYAQVVPM
ENINLHFTGDFHAISAANNLLAALVDNHIYQGNALQFDTVSWRRCIDMNDRALRRLTLEN
NTHTGFDITVASEVMAIFCLATDLADLTSRLGRIQVGLSTAGSPILASDLQAEGAMAALL
KDAFQPNLVQTLEGSPALVHGGPFANIAHGCNSLVATQTALRLADYVVTEAGFGADLGAE
KFMDIKCRQSGLRPDVAVIVATVRALKYHGGVDVADLNRENLAALQVGVANLRKHVTNLR
QHFNLPVVVALNHFTADTEAEIALVQAAVQALGASLHVCHHWAQGAAGAIALAHAVADQA
QQASTPSLLYADDLSLTEKLDTIAQKLYGASGVTFSALAQQQLARLAEVSQGLPVCVAKT
QYSFSCDPKLRNVPADHVLHVRELRLSRGAGFVVAICGDVMTMPGLPKQPASTRISVDAD
GRIQGLS