Protein Info for GFF1021 in Methylophilus sp. DMC18

Annotation: HTH-type transcriptional regulator SsuR

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 308 PF00126: HTH_1" amino acids 4 to 64 (61 residues), 58.5 bits, see alignment E=5.3e-20 PF03466: LysR_substrate" amino acids 89 to 292 (204 residues), 157.9 bits, see alignment E=2.3e-50

Best Hits

Swiss-Prot: 48% identical to SSUR_BURCJ: HTH-type transcriptional regulator SsuR (ssuR) from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)

KEGG orthology group: K13634, LysR family transcriptional regulator, cys regulon transcriptional activator (inferred from 71% identity to mfa:Mfla_1669)

MetaCyc: 50% identical to DNA-binding transcriptional dual regulator CysB (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Cys regulon transcriptional activator CysB" in subsystem Cysteine Biosynthesis or DNA-binding regulatory proteins, strays

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (308 amino acids)

>GFF1021 HTH-type transcriptional regulator SsuR (Methylophilus sp. DMC18)
MKFHQLRYVHEVVRQHLNISAASEALHTSQPGVSKQIQLLEEELGLQIFQRNGKRLIGVT
EPGRKIVELAARVMLDMQNIKRVGDEYSREDSGELTIATTHTQARYRLPAAVKQFMAQYP
NVKLTIHQGNPSQVTEQVAAGEADIGIATENISKDERLSCLPCYSWNRCLVVPSQHPLLE
KKTISLKDLVNYPLITYDFAFTGGTLVSKVFHDAGLQPNVVFTAIDADVIKTYVNLGLGI
GLIANMAYEETRDAPLKSLDCSHLFPDSTTYLGVRRDAFLRDYMLNFITMLSPQYDKRSV
QRALHQDL