Protein Info for GFF1017 in Methylophilus sp. DMC18

Annotation: Thioredoxin-dependent 5'-adenylylsulfate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00434: phosophoadenylyl-sulfate reductase
  • amino acids 41 to 249 (209 residues), 168.4 bits, E=2e-53
• PF01507: PAPS_reduct
  • amino acids 51 to 224 (174 residues), 156.1 bits, E=5e-50
• TIGR02055: adenylylsulfate reductase, thioredoxin dependent
  • amino acids 55 to 248 (194 residues), 256.8 bits, E=1.3e-80

Best Hits

Swiss-Prot: 52% identical to CYSH_BURCE: Thioredoxin-dependent 5'-adenylylsulfate reductase (cysH) from Burkholderia cepacia

KEGG orthology group: K00390, phosphoadenosine phosphosulfate reductase [EC: 1.8.4.8] (inferred from 77% identity to meh:M301_1202)

MetaCyc: 59% identical to assimilatory adenylyl-sulfate reductase (Allochromatium vinosum)
Adenylyl-sulfate reductase (glutathione). [EC: 1.8.4.9]

Predicted SEED Role

"Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) / Adenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.10)" in subsystem Cysteine Biosynthesis (EC 1.8.4.10, EC 1.8.4.8)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (7/9 steps found)
• assimilatory sulfate reduction I (3/4 steps found)
• assimilatory sulfate reduction II (2/3 steps found)
• assimilatory sulfate reduction IV (2/4 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (6/10 steps found)
• selenate reduction (2/5 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.8.4.10 or 1.8.4.8 or 1.8.4.9

Protein Sequence (252 amino acids)

>GFF1017 Thioredoxin-dependent 5'-adenylylsulfate reductase (Methylophilus sp. DMC18)
MSFGVSMLKPAASNPATRPELTEALVASVQAKRAQVVALLQSAAAEFPAITFANSYGAED
MVLTDLIAKEALPIEIFSLDTGRLPAETYTLMGEVEHTYATKPVVFFPKHEAVEQYVRSQ
GINAFYDSIELRKACCHMRKVEPLQRALAGKQAWITGMRAEQATTRSNLPVREFDAGNKL
EKFNPLSDWTEKEVWAYIRLHEVPYNALHDQFYPSIGCAPCTRAVAMGEDIRAGRWWWED
PTSKECGLHVKK