Protein Info for GFF1000 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Butyryl-CoA dehydrogenase (EC 1.3.99.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02770: Acyl-CoA_dh_M
  • amino acids 145 to 243 (99 residues), 84.6 bits, E=6.8e-28
• PF00441: Acyl-CoA_dh_1
  • amino acids 255 to 404 (150 residues), 157.5 bits, E=4.2e-50
• PF08028: Acyl-CoA_dh_2
  • amino acids 271 to 386 (116 residues), 65.3 bits, E=1.1e-21

Best Hits

KEGG orthology group: K00249, acyl-CoA dehydrogenase [EC: 1.3.99.3] (inferred from 68% identity to rme:Rmet_4410)

Predicted SEED Role

"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)

MetaCyc Pathways

• 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Fatty acid metabolism
• Propanoate metabolism
• Valine, leucine and isoleucine degradation
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.2, 1.3.99.3

Use Curated BLAST to search for 1.3.99.2 or 1.3.99.3

Protein Sequence (410 amino acids)

>GFF1000 Butyryl-CoA dehydrogenase (EC 1.3.99.2) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MDFTIDPDTVAIAEAVLRFVEREVLPLQQRHHDLLGSERTLFDASGRYVPEALALRQQVR
KRSAELGFYTLFGDEKLGGGGQGAQVMAHVQESLNHCVGPAQPLVQTVVLPSPFTNGLSP
VLRHLKPDIFAQYRDDIASGDKTLCFGLSEPDAGSDVFGMQTRAVRDGDEWVLTGTKQWI
TNSPYADYAMVFALTDPEAAARHKGGVTGFFVDTRASGFSVPRIINTMGHLGGDTGVIVL
DGVRVRDDHRLGDVGHGLAVAMDGVNAGRMGMAASCLGLARWALDQAVDYAKVRKTFGVP
IAEHQAIQLMLADSAMDIYAAKTMIQNCAWRLDSGRSANAQVSMVKAFSTEMLGRVMDRS
IQIHGGMGLTNELRLEEGYRFARVMRIPDGTGEIQRRTVARQLLTGQADL