Protein Info for GFF10 in Variovorax sp. SCN45

Annotation: Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 384 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 68 to 384 (317 residues), 166.1 bits, see alignment E=5.1e-53 PF16576: HlyD_D23" amino acids 78 to 310 (233 residues), 149.7 bits, see alignment E=1.5e-47 PF13533: Biotin_lipoyl_2" amino acids 93 to 138 (46 residues), 37.5 bits, see alignment 3.1e-13 PF13437: HlyD_3" amino acids 205 to 301 (97 residues), 51.9 bits, see alignment E=2.3e-17

Best Hits

KEGG orthology group: None (inferred from 47% identity to llo:LLO_3381)

Predicted SEED Role

"Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (384 amino acids)

>GFF10 Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family (Variovorax sp. SCN45)
MKKKSLYQITPARIFFGVLTCAALCVDSFDNNAHAATVAATAPAPMLVRQGNRLTVPEGS
PLRDRLRVATVGEQASPHDVSLPATVEANPASTVNVLPPLTGKLAELKVRLGDTVKRGQV
LATMTSPDLAQAVSDAQKARDALDLAQRALVRARGVNEAGSNAAKDVEAAQSAVAQQTAE
LRRSETRLRSLGADGSGAQAGQLLKIVAPISGTVTALNAAAGANLNDATATLMTVANLDS
VWVTVNVPENVVGAVAPGQNVTVSLAAFPGRRFTGKVGFVSAVLEADTRRAKARIVFANP
EGLFKPNMYATAVLGVPQAAQPQVPASALLMNNDAVSVFVEAEPWTFVRRMVELGREDGD
QVRIRSGLNAGERVVVRGGVLLND