Protein Info for GEMAOFIL_04534 in Pseudomonas lactucae CFBP13502

Annotation: High-affinity gluconate transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 transmembrane" amino acids 9 to 26 (18 residues), see Phobius details amino acids 32 to 50 (19 residues), see Phobius details amino acids 61 to 80 (20 residues), see Phobius details amino acids 108 to 136 (29 residues), see Phobius details amino acids 144 to 162 (19 residues), see Phobius details amino acids 182 to 209 (28 residues), see Phobius details amino acids 230 to 249 (20 residues), see Phobius details amino acids 269 to 288 (20 residues), see Phobius details amino acids 301 to 323 (23 residues), see Phobius details amino acids 339 to 358 (20 residues), see Phobius details amino acids 365 to 384 (20 residues), see Phobius details amino acids 390 to 413 (24 residues), see Phobius details amino acids 425 to 449 (25 residues), see Phobius details PF02447: GntP_permease" amino acids 10 to 449 (440 residues), 602.5 bits, see alignment E=4.5e-185 TIGR00791: transporter, gluconate:H+ symporter (GntP) family" amino acids 14 to 450 (437 residues), 539.3 bits, see alignment E=4e-166 PF03600: CitMHS" amino acids 23 to 394 (372 residues), 49.8 bits, see alignment E=2.9e-17

Best Hits

Swiss-Prot: 84% identical to GNUT_PSEAE: Gluconate permease (gnuT) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03299, gluconate:H+ symporter, GntP family (inferred from 99% identity to pfs:PFLU4810)

MetaCyc: 37% identical to fructuronate transporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-81; TRANS-RXN0-209

Predicted SEED Role

"Gluconate transporter family protein" in subsystem D-gluconate and ketogluconates metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (450 amino acids)

>GEMAOFIL_04534 High-affinity gluconate transporter (Pseudomonas lactucae CFBP13502)
MFGMSHESYLLLDAVVTIIGLIVLITKFKVHPFIALIIAAGFLGLTSGMPVDKIIKAFQD
GFGGVLGFVGIILALGTMLGKMMAESGGADQIAQTLIRAFGKEKVQWAMMFAAFLVGIPL
FFEIGFVLLIPLVFIVARRTGVSLIKIGIPLLAGLSAVHGLVPPHPGPLLAIGVFGADIG
KTILYGLIVALPTAIIAGPIFGTFIAKYIPGNPSQELVDQLARETDNKSLPSFGITLVTV
LLPVFLMLLKTFADIAFAEGNVVRNWMDMIGHPITALLLALLLSLYTFGTRQGIHAKQIL
KLLDASLAPTAAIILIIGAGGGFKQMLVTSGVGDVIGHMAVNAQINPILLAWLVAAVIRV
ATGSATVATITGAGIVVPVVGMIPGVNRELLVLATGAGSLILSHVNDAGFWLVKQYFNMT
VAETFKTWTAMETILSIVALIFIMLLSLVV