Protein Info for GEMAOFIL_00730 in Pseudomonas lactucae CFBP13502

Annotation: Epoxyqueuosine reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 354 TIGR00276: epoxyqueuosine reductase" amino acids 15 to 351 (337 residues), 505.5 bits, see alignment E=3e-156 PF08331: QueG_DUF1730" amino acids 63 to 141 (79 residues), 85.7 bits, see alignment E=1.6e-28 PF13484: Fer4_16" amino acids 194 to 258 (65 residues), 82.5 bits, see alignment E=2.9e-27

Best Hits

Swiss-Prot: 84% identical to QUEG_PSEAE: Epoxyqueuosine reductase (queG) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 96% identity to pfs:PFLU0514)

MetaCyc: 59% identical to epoxyqueuosine reductase (Escherichia coli K-12 substr. MG1655)
RXN-12104 [EC: 1.17.99.6]

Predicted SEED Role

"Iron-sulfur cluster-binding protein"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.99.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (354 amino acids)

>GEMAOFIL_00730 Epoxyqueuosine reductase (Pseudomonas lactucae CFBP13502)
MPAITFDLPALAQSIKDWGRELGFQQVGISGLDLAEHEQHLQRWLDAGYHGEMDYMGAHG
SKRSHPEELVPGTLRVVSLRMDYLPGDTHMAQLLAQPEKAYISRYALGRDYHKLIRKRVQ
QLADRIQAQIGPFGFRAFVDSAPVLEKAIAEQAGLGWIGKNTLVLNRKAGSYFFLSELFV
DLPLPLDEPHRTEHCGRCTACLDICPTNAFVGPYVLDARRCISYLTIELKSAIPEDLRAL
IGNRVFGCDDCQIVCPWNRFARSTAEGDFMPRHNLDNAELAELFMWDEDKFLSSTEGSPL
RRAGYERWLRNLAVGLGNAPSSIVVLEALKARRDYPSELVREHVEWALNQHATR