Protein Info for Echvi_4645 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: GTP-binding protein LepA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00231: small GTP-binding protein domain
  • amino acids 16 to 188 (173 residues), 71.3 bits, E=8.2e-24
• TIGR01393: elongation factor 4
  • amino acids 16 to 608 (593 residues), 981.4 bits, E=1.4e-299
• PF00009: GTP_EFTU
  • amino acids 16 to 193 (178 residues), 189.6 bits, E=8.7e-60
• PF03144: GTP_EFTU_D2
  • amino acids 217 to 287 (71 residues), 35 bits, E=3.2e-12
• PF00679: EFG_C
  • amino acids 411 to 492 (82 residues), 62.9 bits, E=4.8e-21
• PF06421: LepA_C
  • amino acids 500 to 606 (107 residues), 176.1 bits, E=3.7e-56

Best Hits

Swiss-Prot: 77% identical to LEPA_CYTH3: Elongation factor 4 (lepA) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 79% identity to dfe:Dfer_5632)

MetaCyc: 56% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (7/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (11/14 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See L0G5I6 at UniProt or InterPro

Protein Sequence (608 amino acids)

>Echvi_4645 GTP-binding protein LepA (Echinicola vietnamensis KMM 6221, DSM 17526)
MRRVFTIFADRMDMQKIRNFCIIAHIDHGKSTLADRLLQFTNTVTEREMQDQLLDNMDLE
RERGITIKSHAIQMKYTYQEEEYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDASQGV
EAQTISNLYLAMEHDLEIIPVLNKIDLPGAEPEVVADEVIDLIGCDREDIILASGKEGTG
IEDILNAVVEKIPAPEGNAEEPLQAMIFDSVYNPFRGVEVLFRVFNGTINKGDKIKFVNT
GREYDADEIGVLGIVQHPQKTISAGNVGYLISGIKVAKEVKVGDTITHIKRPCAKAIKGF
ENVKPMVFAGIYPVDTTEFEELRASMEKLQLNDASLVWEPETSAALGFGFRCGFLGMLHM
EIIQERLEREFGMTVITTVPSVQFKALMNDDTIKLINAPSDMPDPNLFRHIEEPYVHASI
ITKSDYVGPVIQLCMEKRGQIKNQVYLTADRVELTFDMPLAEIVFDFFDKLKTISRGYAS
LDYDLRENKPSKMVRLDVMLNGEVVDALSAVVHRDKAYEWGKRLCEKLKELVPRQMFEIA
IQAAIGTKIIARETVKAMRKNVLAKCYGGDISRKRKLLDKQKKGKKRMRQVGNVEVPQEA
FMAVLKLD