Protein Info for Echvi_4635 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: endopeptidase Clp ATP-binding regulatory subunit (clpX)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 407 TIGR00382: ATP-dependent Clp protease, ATP-binding subunit ClpX" amino acids 3 to 394 (392 residues), 562 bits, see alignment E=4.2e-173 PF06689: zf-C4_ClpX" amino acids 5 to 39 (35 residues), 52.5 bits, see alignment 1.3e-17 PF00493: MCM" amino acids 60 to 185 (126 residues), 35.9 bits, see alignment E=1.5e-12 PF07724: AAA_2" amino acids 104 to 299 (196 residues), 106.1 bits, see alignment E=7.3e-34 PF07728: AAA_5" amino acids 106 to 183 (78 residues), 22.7 bits, see alignment E=3.1e-08 PF00004: AAA" amino acids 107 to 224 (118 residues), 54.3 bits, see alignment E=6.9e-18 PF10431: ClpB_D2-small" amino acids 306 to 384 (79 residues), 41.8 bits, see alignment E=3.1e-14

Best Hits

Swiss-Prot: 69% identical to CLPX_BACTN: ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K03544, ATP-dependent Clp protease ATP-binding subunit ClpX (inferred from 78% identity to mtt:Ftrac_1139)

MetaCyc: 54% identical to ATP-dependent Clp protease ATP-binding subunit ClpX (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpX" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G675 at UniProt or InterPro

Protein Sequence (407 amino acids)

>Echvi_4635 endopeptidase Clp ATP-binding regulatory subunit (clpX) (Echinicola vietnamensis KMM 6221, DSM 17526)
MAQVTCSFCGRNKKDVDLMVSGISAHICNFCIDQAYQILGEEEKTKKQEKKPKFKLRKPK
ELTEYLNNYVIGQDEAKKVLSVAVYNHYKRLLQKDNKDDVKIEKSNIIMVGDTGTGKTYL
AKTLAKILEVPFCIADATVLTEAGYVGEDVESILTRLLQSADYNLEAAERGIVYIDEIDK
IARKSDNPSITRDVSGEGVQQAMLKLLEGTTVNVPPQGGRKHPDQKMIAVNTEDILFICG
GAFDGISRHIANRLNTQPLGFSKKTESDVVDRNNLLQYVTAPDLKSFGLIPELIGRLPVL
THLDPLDKETLKSILTEPKNALTKQYIKLMDMEGVKLEFEESGIDYIVEKAVEYNLGARG
LRSICEAIITDAMYELPSDQETKELVVNRKYAQRQFDKSKFKKLQVA