Protein Info for Echvi_4081 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Malic enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00390: malic
  • amino acids 20 to 115 (96 residues), 84.7 bits, E=1.1e-27
  • amino acids 116 to 151 (36 residues), 24.3 bits, see alignment (E = 3.8e-09)
• PF03949: Malic_M
  • amino acids 166 to 357 (192 residues), 80.4 bits, E=2.1e-26
• PF01515: PTA_PTB
  • amino acids 430 to 748 (319 residues), 320.2 bits, E=2.6e-99

Best Hits

KEGG orthology group: K00029, malate dehydrogenase (oxaloacetate-decarboxylating)(NADP+) [EC: 1.1.1.40] (inferred from 71% identity to mtt:Ftrac_3335)

Predicted SEED Role

"NADP-dependent malic enzyme (EC 1.1.1.40)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 1.1.1.40)

MetaCyc Pathways

• gluconeogenesis I (11/13 steps found)
• C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
• methylgallate degradation (2/6 steps found)
• protocatechuate degradation I (meta-cleavage pathway) (3/8 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (7/14 steps found)
• superpathway of vanillin and vanillate degradation (3/10 steps found)
• syringate degradation (3/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of terpenoids and steroids
• Carbon fixation in photosynthetic organisms
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.40

Sequence Analysis Tools

See L0G4P2 at UniProt or InterPro

Protein Sequence (757 amino acids)

>Echvi_4081 Malic enzyme (Echinicola vietnamensis KMM 6221, DSM 17526)
MAIKIRKEDALGYHSQGSPGKIEVVPTKPLSSQLDLALAYSPGVAEPCLEIAKQKENVYK
YTAKGNLVAVISNGTAVLGLGDIGPEAGKPVMEGKGVLFKKFAGIDVFDIEIDEKDPQKL
IQIIKSLEPTFGGINLEDIKAPECFEIEQALKQQMKIPVMHDDQHGTAIISGAALLNALE
IVGKKIEEIRLVVCGAGAAAVSCTRFYMSLGVKRENLVMVDIEGVIRSDRPDLDDIRKEF
STSRESLHTLSDAMKGADVFLGLSAGNIVSPEQIKSMAENPIVFALANPHPEIDYDLATA
TREDLIMATGRSDFPNQVNNVLGFPYIFRGALDVRATAINEEMKLATAHAIAKLAKEPVP
EIVNKAYGDDQLGFGRNYLIPKPLDPRLITTIAPAVAKTAMDTGVAQTAITDWETYELEL
QERIGIDQRLMSRVIARAKKHPKRVVFAEADNTKILKAAQLINDERICDPILLGNRETIL
KLIEEHSLDLSNVPIIDPYEEPERLEKFGNLLYNKRQRKGLTPFEAQKLMRDRTYFGAMM
VETGEADALISGLTKDYPKTILPALHVIGVKKDVDRVAGMYIMNSDKGPYFFADTTVNVD
PTADQIVEIIGLTADAVKFFDLEPKVAVLSYSNFGSAKGNTPTKTALATAKAKEKFPDLI
IEGEMQANVAINEAIQKENYPFSALANKKANTLIFPNLSSGNIAYKLLAEIGNAEAIGPV
LLGMNKPVHILQLGSSIREIINMVAIAVVDAQSHDNI