Protein Info for Echvi_4061 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: histidyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 TIGR00442: histidine--tRNA ligase" amino acids 8 to 444 (437 residues), 402.9 bits, see alignment E=8.1e-125 PF13393: tRNA-synt_His" amino acids 11 to 348 (338 residues), 122 bits, see alignment E=3.2e-39 PF03129: HGTP_anticodon" amino acids 375 to 456 (82 residues), 56.5 bits, see alignment E=2.6e-19

Best Hits

Swiss-Prot: 61% identical to SYH_CYTH3: Histidine--tRNA ligase (hisS) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K01892, histidyl-tRNA synthetase [EC: 6.1.1.21] (inferred from 62% identity to dfe:Dfer_1143)

Predicted SEED Role

"Histidyl-tRNA synthetase (EC 6.1.1.21)" (EC 6.1.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G4M4 at UniProt or InterPro

Protein Sequence (457 amino acids)

>Echvi_4061 histidyl-tRNA synthetase (Echinicola vietnamensis KMM 6221, DSM 17526)
MSIQKPSLPKGTRDFGPAQMARRNYILDTIKGTFRLFGYQQLETPSMENLSVLTGKYGDE
GDQLLFKILNSGDFLKKVRPEDLEKGAVQVLPKVAEKGLRYDLTVPFARYVVMNRNEITF
PFKRYQIQPVWRADRPQKGRYREFYQCDADVVGTDSLICEAEILLMIRKVFAAFGLSDYD
IKINNRKILTGISEVIGAPGKEGELCVAIDKLDKIGWEKVQEELVQRGFHQDAIQKLTPI
IKLEGDNNNKLEFLKDFLATSEEGLKGVAELEEVFSLLEGFGENQAHVVFDVVLARGLSY
YTGAIFEVKVNNVSIGSVSGGGRYDNLTGVFGLEGVSGVGFSFGVDRIYDVLEELGLFPE
EEMQATSVMIGYFDEKGRNYGLKVLNGLRKAGLSAEIYPDVAKVKKQFNYADKKQIPYVM
MIGDDEIAAHKVALKNLKTGTQEMHTLEEAIGIIKAS