Protein Info for Echvi_4039 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: aconitate hydratase 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01341: aconitate hydratase 1
  • amino acids 19 to 922 (904 residues), 1327.8 bits, E=0
• PF00330: Aconitase
  • amino acids 73 to 593 (521 residues), 531.2 bits, E=2.7e-163
• PF00694: Aconitase_C
  • amino acids 723 to 849 (127 residues), 141.8 bits, E=1.7e-45

Best Hits

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 70% identity to gfo:GFO_1390)

Predicted SEED Role

"Aconitate hydratase (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.3)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle III (animals) (8/10 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (19/26 steps found)
• nitrogen remobilization from senescing leaves (6/8 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (10/14 steps found)
• glyoxylate cycle (4/6 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (6/9 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• superpathway of glyoxylate bypass and TCA (8/12 steps found)
• reductive TCA cycle I (7/11 steps found)
• TCA cycle VII (acetate-producers) (5/9 steps found)
• mixed acid fermentation (10/16 steps found)
• methylaspartate cycle (11/19 steps found)
• reductive TCA cycle II (5/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3

Use Curated BLAST to search for 4.2.1.3

Sequence Analysis Tools

See L0G5F0 at UniProt or InterPro

Protein Sequence (925 amino acids)

>Echvi_4039 aconitate hydratase 1 (Echinicola vietnamensis KMM 6221, DSM 17526)
MSLDPYHIKKQLDTAKGSLNYWSLAALQEQGHKINELPFSIRILLENALRNYDDFAITKE
HTETLLNWKPEASDKDVPYKPARVLMQDFTGVPAVVDIASLRAEAVRKGKDPQKINPLIP
VDLVVDHSVQVDYFGTNYSYKKNVDVEYERNGERYEFLKWAQKSFDNFSVVPPGMGICHQ
VNLEYLAKGVIERDGNVFPDTLVGTDSHTPMVNGIGVVGWGVGGIEAEAALLGQPIYFIM
PQVVGLKLTGELPLGTTATDMVLTITELLRKHGVVGKFVEVFGPGLDTLTVPDRATISNM
SPEFGCTVTYFPIDDRTLDYMSKTNRSKDQIKLVEDYAKANMLWREDEESVKYSSLVELD
LGTVEPTVSGPKRPQDKILVRNFKEKFGELLEEVHGREYIPIDKRDKSRWFSEGGGQPVD
KPGDLPEDVEIATKTKNGLKTVEVKINNEEFALSDGSIVIAAITSCTNTSNPSVMIGAGL
VAQKARERGLDVKPWVKTSLAPGSKVVTDYLEASGLLDDLEALRFHVVGYGCTSCIGNSG
PLPKHIAHAVEENDLVVASVLSGNRNFEARVHPQVKMNYLMSPMLVVAYALAGRVDVDLN
EEPLGFDPNLEPVYLKDIWPSNDEIFEVMGKVLSPGDFDKNYGEIFEGNEQWKNLQAPSD
KVYNWSEKSTYIKEAPFFQGLSNEVPEPQNIQGARVLLKLGDSITTDHISPAGAFAESSP
AGQYLVGRGVEKKDFNSYGSRRGNDEVMVRGTFANVRIKNQLASREGGYTTHIPSGEEMT
VFEASEKYQKDDTPLVVLAGKEYGSGSSRDWAAKGTTLLGIHAVIAESYERIHRSNLVGM
GVLPLQFAEGQSASSLGLDGKEEITIEGITEGLTPLKNLKATAKKDGGAVVNFDVVCRLD
SEVEIAYYKNGGILHYVLREFLKQD