Protein Info for Echvi_3957 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: DNA ligase, NAD-dependent

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 820 PF01653: DNA_ligase_aden" amino acids 8 to 320 (313 residues), 393.7 bits, see alignment E=1.9e-121 TIGR00575: DNA ligase, NAD-dependent" amino acids 16 to 493 (478 residues), 603.9 bits, see alignment E=1.9e-185 PF03120: DNA_ligase_OB" amino acids 324 to 400 (77 residues), 106.1 bits, see alignment E=2.1e-34 PF03119: DNA_ligase_ZBD" amino acids 409 to 435 (27 residues), 44.5 bits, see alignment (E = 3.4e-15) PF12826: HHH_2" amino acids 659 to 721 (63 residues), 78.5 bits, see alignment 1.1e-25 PF14520: HHH_5" amino acids 663 to 710 (48 residues), 27.9 bits, see alignment 8.2e-10 PF00533: BRCT" amino acids 744 to 818 (75 residues), 35.1 bits, see alignment E=3.8e-12

Best Hits

Predicted SEED Role

"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G3R6 at UniProt or InterPro

Protein Sequence (820 amino acids)

>Echvi_3957 DNA ligase, NAD-dependent (Echinicola vietnamensis KMM 6221, DSM 17526)
MSTIPKPEAQKRIAELTATINHHNQLYYQKDQSEISDFEFDKLLEELMLLEEQFPEFQDK
NSPTQHVGGTITKEFETVEHETRMLSLGNTYSKEELIAFDERVAKGLGHRQYSYCCELKF
DGVAISLVYEKGELVRAVTRGDGYRGDNVTANVKTIKNIPLYLQGDDVPDKFEIRGEIFL
PVKEFEKINTEREANGEALLANPRNTASGTLKMQDSSIVAKRRLNCYFYQLLGDQIGVDQ
HDQAMDRLEKWGFNISPTYKKCTDINAVFEYIESWREKRHSLPLETDGVVLKVNDYAQRE
ELGFTAKIPRWAIAYKYKAESAESTLMSVTYQVGRTGAITPVANLSPVLLAGTTVKRASL
HNANEIMRLDLHHGDVVFVEKGGEIIPKITAVAVEKRKADAKPIAYITHCPECGTPLERK
EGEAKHFCPNTASCPPQVLGRIEHFVHKRAMDIDSMGTERIRALINQGYIEHPADLYELE
AKKDQLLGLEINAEQYEKSSDGYLYVTLRKALFAITDGISLSAIDRYLSDTEALEQHQKL
AQFPDFIQQQNKKVALNLAALDKLKDTLSDLPHDQIEDFLPVSAVMAMFVGNQCSMEKLQ
LLSQQKNTVHDIVLTFDFELHQDQEDKIKKLKGNTFQEGVISNMLAGIQASKNQPFEKVL
FALGIRNIGENTAQLLARHFKTIENLTQASTEQLLDINGVGETLVGSIREFFSQEENKQN
IQRLKAHGLHFEIKAEDLPEQKGNALEGLKVLASGKFEHFKRDEIIEAISAHGGTYLKSV
SKNLDLIIEGADMGPSKKEKAQKLGIKMISENEFLSMIAD