Protein Info for Echvi_3848 in Echinicola vietnamensis KMM 6221, DSM 17526

Updated annotation (from data): N-succinylornithine aminotransferase (EC 2.6.1.81)
Rationale: Important for fitness in most defined media, except when arginine or citrulline is provided. Similar to acetylornithine aminotransferases (such as argD from B. subtilis), but Bacteroidetes use succinylated intermediates for arginine synthesis (see PMC7311316).
Original annotation: Ornithine/acetylornithine aminotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00202: Aminotran_3
  • amino acids 12 to 375 (364 residues), 311.4 bits, E=4.2e-97

Best Hits

Swiss-Prot: 42% identical to ARGD_METKA: Acetylornithine aminotransferase (argD) from Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

KEGG orthology group: K00818, acetylornithine aminotransferase [EC: 2.6.1.11] (inferred from 60% identity to dfe:Dfer_4419)

Predicted SEED Role

"Acetylornithine aminotransferase (EC 2.6.1.11)" in subsystem Arginine Biosynthesis extended (EC 2.6.1.11)

MetaCyc Pathways

• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-ornithine biosynthesis I (5/5 steps found)
• superpathway of arginine and polyamine biosynthesis (12/17 steps found)
• L-arginine degradation II (AST pathway) (1/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.11

Use Curated BLAST to search for 2.6.1.11 or 2.6.1.81

Sequence Analysis Tools

See L0G5F2 at UniProt or InterPro

Protein Sequence (381 amino acids)

>Echvi_3848 N-succinylornithine aminotransferase (EC 2.6.1.81) (Echinicola vietnamensis KMM 6221, DSM 17526)
MKPFDVYPLIDVTPVKASGSTIWDDQGNEYLDLYGGHAVISIGHSHPHYTKRIKEQLDNI
AFYSNSVQIPIQKELATKLGQLSGYPDYDLFLCNSGAEANENALKLASFETGKKGFIAFT
KGFHGRTSGAVALTDNPKIIAPFNAHEGVHILPFNDLEAVEKQLATGTIAGVIVEGIQGV
GGIQVPDPAFLLGLSALTKQYGAKLILDEVQSGYARSGKFFAHQWVEGLKPDLITVAKGM
GNGFPIGGVLISPEFKASHGLLGTTFGGNHLACAAALAVLEVIDEENLITAAAENGKAIM
AALEKVAGVTEVRGKGLMIGFDLATEAGPVRAALIHEHKIFTGSAGGKHTIRLLPPLNIE
PKALTLFLEKLETVLNVKQST