Protein Info for Echvi_3808 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: DNA topoisomerase III, bacteria and conjugative plasmid

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 780 TIGR01056: DNA topoisomerase III" amino acids 1 to 692 (692 residues), 592.4 bits, see alignment E=7e-182 PF01751: Toprim" amino acids 2 to 132 (131 residues), 40.3 bits, see alignment E=4.3e-14 PF01131: Topoisom_bac" amino acids 150 to 574 (425 residues), 388.7 bits, see alignment E=5.4e-120 PF13342: Toprim_Crpt" amino acids 654 to 710 (57 residues), 32.8 bits, see alignment 8.4e-12

Best Hits

KEGG orthology group: K03169, DNA topoisomerase III [EC: 5.99.1.2] (inferred from 59% identity to pdi:BDI_1687)

Predicted SEED Role

"DNA topoisomerase III (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G5C1 at UniProt or InterPro

Protein Sequence (780 amino acids)

>Echvi_3808 DNA topoisomerase III, bacteria and conjugative plasmid (Echinicola vietnamensis KMM 6221, DSM 17526)
MKVCIAEKPSVAREIAQVIGAKNRKDGYFEGNGYQVTWTFGHFCSLYPPEDYDPTWKRWD
LYTLPMLPKRFETKIIPDNGVKKQFKIIRDLVNKAQVVINCGDAGQEGELIQRWVLKQAN
YQGAVQRLWISSLTTESIQAGFENLKPAEEFDNLYYAGSSRAIGDWLLGMNATRLYTLKY
GGYKQVLSIGRVQTPTLAMLVNRHQEIEQFKPEPYWELQTTYRDTTFHSTEGKFLKKEDG
EALLGQITGKDLFITAIDQKEGKEYAPKLFDLTSLQVYCNNKFNLTADNTLKTVQRLYEL
KVVTYPRVDTTFLPNDMYPKISGILKGLGQYHSFTAPLLAKKIRKSPKVFNDKKVTDHHA
IIPTGEEKQLGHTEQKVYDIIVRRFLAAFYPDCKVAKTQVTGEIEQTTFLAKGKEILEEG
WRALFAKDNKKEKDDGDSKEDEEEGILPTFEKGEHGPHHPTLKEKITKPPKNYTEASLLR
AMETAGKQVEDDELRDLMKANGIGRPSTRASIIETLFRRKYIERRKKLVVPTEMGIQLIG
TIQNELLKSAELTGQWEKQLKEIEEGAFSAIQFIANMKKMVAELVSEVKQEHNKPRLASP
FASDNQKKSTPSASKKKTSKAASKGLSEVTCPKCGQGNIIKGKTAYGCSQWKNGCDLRLP
MKVKGKKLTDKQVQRLIEKGATTKLKGFLMDGEKVEGILKLTDSFAINFDLKAQAPTKPT
PPQMPPCPKCKKGTIIKGKTAYGCSEWKSGCDFRYAFKSIKEMANGKPLTKELVLSIISQ