Protein Info for Echvi_3769 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02746: MR_MLE_N
  • amino acids 19 to 111 (93 residues), 84.8 bits, E=5.3e-28
• PF13378: MR_MLE_C
  • amino acids 133 to 359 (227 residues), 220.8 bits, E=1.8e-69

Best Hits

Swiss-Prot: 57% identical to DGOD_PECAS: D-galactonate dehydratase (dgoD) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K01684, galactonate dehydratase [EC: 4.2.1.6] (inferred from 79% identity to fbc:FB2170_02045)

MetaCyc: 55% identical to D-galactonate dehydratase (Escherichia coli K-12 substr. MG1655)
Galactonate dehydratase. [EC: 4.2.1.140, 4.2.1.6]

Predicted SEED Role

"Gluconate dehydratase (EC 4.2.1.39)" in subsystem Entner-Doudoroff Pathway (EC 4.2.1.39)

MetaCyc Pathways

• Entner-Doudoroff pathway III (semi-phosphorylative) (7/9 steps found)
• D-galactonate degradation (1/3 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (5/9 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Pentose phosphate pathway

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.140 or 4.2.1.39 or 4.2.1.6

Sequence Analysis Tools

See L0G4P1 at UniProt or InterPro

Protein Sequence (386 amino acids)

>Echvi_3769 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily (Echinicola vietnamensis KMM 6221, DSM 17526)
MSNDLKIERIELFKVPPRWLFVKITTKSGLVGWGEPVIEGKADTVAACVREMEQYLIGRG
AHEIEDIWQVLYRGGFYRGGPILMSALSGIDQALWDIKGKHLNVPVYELLGGAVRQKMKM
YCWIGGDHPEVVLEQAQEKVDAGYTAVKMNATGEMDWVSSVKEVKKVVENIKLIRQHFGD
SLDVGLDFHGRVHKPMVKRLIDELSPFDPLFIEEPVLAENNDALGHIYRYSAIPIATGER
MFSRWDFKEILHQGVVDIIQPDLSHAGGISEVRRIATMAEAYDITIAPHCPLGPISLASA
LHVDFVSANAFIQESSLGIHYNQGFDLLDYVKNPEVFDLKEGYIDLFDRPGLGVEMDEER
LKEGQKIGHHWANPIWRNADGSFAEW