Protein Info for Echvi_3716 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: rRNA methylases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 253 PF22435: MRM3-like_sub_bind" amino acids 5 to 88 (84 residues), 61.5 bits, see alignment E=8.6e-21 PF00588: SpoU_methylase" amino acids 106 to 240 (135 residues), 105.8 bits, see alignment E=2.1e-34

Best Hits

KEGG orthology group: K03437, RNA methyltransferase, TrmH family (inferred from 52% identity to dfe:Dfer_2725)

Predicted SEED Role

"tRNA (guanosine(18)-2'-O)-methyltransferase (EC 2.1.1.34)" (EC 2.1.1.34)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.34

Use Curated BLAST to search for 2.1.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G3N7 at UniProt or InterPro

Protein Sequence (253 amino acids)

>Echvi_3716 rRNA methylases (Echinicola vietnamensis KMM 6221, DSM 17526)
MISKNTLKFIKSLQQKKFRKQEGAFFVEGTKNVTELLHSDFEVTHLLYTAKYQAQHSQLI
AASKAAAYEVPQKVLEATGSFKTNDAALAVAKLKENQPFDVEAGELAIALDDVRDPGNLG
TIIRIADWYGIQKLILSPQTADFYNPKVLHSSMGSFTRVSFFYADLAPYLSRQALPVFGA
FLEGQDIHRQSLAPEGIILMGNEANGISAPLEKTVSHKLTIPSFGHAESLNVAIATAVIC
DNFRRGKFSRSDV