Protein Info for Echvi_3630 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Entner-Doudoroff aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 222 PF01081: Aldolase" amino acids 8 to 206 (199 residues), 122.9 bits, see alignment E=5.8e-40 TIGR01182: 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase" amino acids 9 to 214 (206 residues), 156.8 bits, see alignment E=2.3e-50

Best Hits

KEGG orthology group: K01625, 2-dehydro-3-deoxyphosphogluconate aldolase / 4-hydroxy-2-oxoglutarate aldolase [EC: 4.1.2.14 4.1.3.16] (inferred from 44% identity to fbc:FB2170_15053)

Predicted SEED Role

"4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16) @ 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14)" (EC 4.1.2.14, EC 4.1.3.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.2.14, 4.1.3.16

Use Curated BLAST to search for 4.1.2.14 or 4.1.3.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G4T4 at UniProt or InterPro

Protein Sequence (222 amino acids)

>Echvi_3630 Entner-Doudoroff aldolase (Echinicola vietnamensis KMM 6221, DSM 17526)
MKFSNSEIIEAMEKTGMIPVFNHSDLEVAKNVMDASYNGGVRVFEFTNRGENALEVFREL
KAYSSRHKGLMLGIGTIFTPKEVEDFIEAGADFIVSPALIPNVAVTATRNDTLWIPGCGT
VTEIFNAREMGAQVIKAFPGNVLGPSFISAVKAVLPSLKIMPTGGVEPTEENLGQWFKAG
VTCVGMGSQLFKKDWIKQKKFDALEKQISEALDTIQRIRNSQ