Protein Info for Echvi_3572 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: DNA gyrase, A subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 842 TIGR01063: DNA gyrase, A subunit" amino acids 9 to 813 (805 residues), 1193.2 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 33 to 472 (440 residues), 550.3 bits, see alignment E=3.2e-169 PF03989: DNA_gyraseA_C" amino acids 506 to 547 (42 residues), 42 bits, see alignment 5e-15 amino acids 555 to 603 (49 residues), 35.2 bits, see alignment 6.9e-13 amino acids 616 to 657 (42 residues), 31.4 bits, see alignment 9.9e-12 amino acids 664 to 711 (48 residues), 43.8 bits, see alignment 1.3e-15 amino acids 717 to 762 (46 residues), 48 bits, see alignment 6.5e-17 amino acids 766 to 812 (47 residues), 53.9 bits, see alignment 9.7e-19

Best Hits

Swiss-Prot: 57% identical to GYRA_BACSU: DNA gyrase subunit A (gyrA) from Bacillus subtilis (strain 168)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 75% identity to mtt:Ftrac_0499)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G3A1 at UniProt or InterPro

Protein Sequence (842 amino acids)

>Echvi_3572 DNA gyrase, A subunit (Echinicola vietnamensis KMM 6221, DSM 17526)
MAEGENENIIPINIEEEMRGAYIDYSMSVIVSRALPDVRDGMKPVHRRILFGMQELGVLH
NKPYKKSARIVGEVLGKYHPHGDSAVYETMVRMAQDWSLRYPLVDPQGNFGSIDGDNAAA
MRYTEARLKRIAEELLTDINKETVDFQLNFDDSLKEPVVLPAKIPALLLNGASGIAVGMA
TNMAPHQLGEVIDGTIAYIENNDITVEELMKHVIAPDFPTGGIIYGYNGVKSAYETGRGR
VVMRGKATIETKDTGKEMIIINEIPYLVNKANMIEKTAQLIQEKKLEGISAIRDESDRRG
MRIVYELKRDAIANVVLNNLYKQTQLQTSFSINNVALVKGRPYTLNLKELIVHYVNHRHE
VVTRRTEYELREAEKRAHILQGYLIALDNLDEVISLIRNSRDPETARTGLMEKFELTEIQ
ARAILDMRLQRLTGMEREKIQKEYEELMLLIEDLKDILEKKERRMEIIKTELSEIKERYN
DERRTTIEHNAEDFSYEDMIPNEEVIITVSHQGYVKRTALKEYRTQGRGGVGSRGVSTKD
DDYTEYLFSASTHNYLLIFTDKGKLFWLKTYAIPEGSKTSKGRPIQNLINIESDDKIRSI
IQVTDLNDEDYIQNNFLVMVTKNGVIKKTTLEQYSRPRSNGIIALNIREDDQLLNVEFTH
GDSHILIAAKSGRAIHFHESAVRPMGRTATGVKAITLSDDKDEVVGMVCVNREEATLLVV
SEKGYGKRSAVEEYRITNRGGKGVKAMNVTDKTGSLVAIKSVIDSDDLMIINKSGIIIRT
PVSGLRIMGRATQGVRLIKLNENDEISSVAKVEQVEEEIEEIQDNADENQNESNDSEKPK
EE