Protein Info for Echvi_3567 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: thiamine biosynthesis protein ThiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 17 to 98 (82 residues), 118.1 bits, E=1.2e-38
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 137 to 588 (452 residues), 717.7 bits, E=2.1e-220
• PF01964: ThiC_Rad_SAM
  • amino acids 139 to 584 (446 residues), 621.3 bits, E=7.8e-191

Best Hits

Swiss-Prot: 78% identical to THIC_PSEAB: Phosphomethylpyrimidine synthase (thiC) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 80% identity to psn:Pedsa_1385)

MetaCyc: 76% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (9/10 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (4/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

See L0G398 at UniProt or InterPro

Protein Sequence (623 amino acids)

>Echvi_3567 thiamine biosynthesis protein ThiC (Echinicola vietnamensis KMM 6221, DSM 17526)
MKTLSQENIESYITTDPFPNSKKVYVSGTLHPIKVAMREIALHDTVSHLDRHETVEKNPP
VTVYDTSGPYTDPAAEIDVKKGLPRLREEWITNTGDVEQLDQLTSAYGQRRLANPALDKL
RFDLVKMPYRAKSGNNVSQMHYAKKGIITPEMEYIAIRENQRIAEQQELTKQHPGQNYGA
NTPKGMITPEFVRDEVAMGRAVIPANINHPEAEPMIIGRNFLVKINANIGNSAIGSSIEE
EVEKAVWACRWGADTIMDLSTGKNIHETREWILRNSPVPIGTVPIYQALEKVNGKAEDLT
WELFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTAKRVTGIVSRGGSIMAKWCLAHHKENF
LYTHFEEICEIMKAYNVTFSLGDGLRPGSIADANDPAQFAELETLGELTKIAWKHDVQTI
IEGPGHIPMHMIKENMDKQLVACGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGCA
MLCYVTPKEHLGLPEKKDVKDGVITYKIAAHAADLAKGHPGAQYRDDALSKARFEFRWED
QFNLSLDPDTARSFHDETLPADGAKIAHFCSMCGPNFCSMKITQDVRDYADKNGLNDQEA
IEEGMQKKAQEFSDKGGEIYLNQ