Protein Info for Echvi_3441 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: pyridoxamine-phosphate oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 211 TIGR00558: pyridoxamine 5'-phosphate oxidase" amino acids 1 to 211 (211 residues), 285.2 bits, see alignment E=1.5e-89 PF12766: Pyridox_oxase_2" amino acids 28 to 111 (84 residues), 36.3 bits, see alignment E=1.1e-12 PF01243: Putative_PNPOx" amino acids 32 to 115 (84 residues), 83.2 bits, see alignment E=1.8e-27 PF10590: PNP_phzG_C" amino acids 170 to 211 (42 residues), 78.6 bits, see alignment 4e-26

Best Hits

Swiss-Prot: 58% identical to PDXH_CUPNJ: Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) from Cupriavidus necator (strain JMP 134 / LMG 1197)

KEGG orthology group: K00275, pyridoxamine 5'-phosphate oxidase [EC: 1.4.3.5] (inferred from 58% identity to reu:Reut_A0815)

MetaCyc: 42% identical to pyridoxine/pyridoxamine 5'-phosphate oxidase (Escherichia coli K-12 substr. MG1655)
Pyridoxal 5'-phosphate synthase. [EC: 1.4.3.5]; 1.4.3.5 [EC: 1.4.3.5]

Predicted SEED Role

"Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 1.4.3.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G0F1 at UniProt or InterPro

Protein Sequence (211 amino acids)

>Echvi_3441 pyridoxamine-phosphate oxidase (Echinicola vietnamensis KMM 6221, DSM 17526)
MDLASIRTEYLLKSLNVNQLEGSPLAQFQLWFDEAVHAKVNEPNAMNLATVNAENRPSAR
IVLLKGVDSGFVFYTNYASNKGHELAQNRFAALTFFWPELQRQIRIEGKTEKVSEAISDT
YFLSRPRGSQIGAWASPQSQQIPNRDFLEQAEKKMLVRFEQEPLRRPPHWGGYRLVPDRV
EFWQGRQSRLHDRIVYSLDDKGKWEKSRLAP