Protein Info for Echvi_3376 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: O-6-methylguanine DNA methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 282 PF00165: HTH_AraC" amino acids 19 to 60 (42 residues), 32.3 bits, see alignment 1.7e-11 PF12833: HTH_18" amino acids 32 to 110 (79 residues), 57.2 bits, see alignment E=3.5e-19 PF02870: Methyltransf_1N" amino acids 119 to 156 (38 residues), 21.8 bits, see alignment 4.6e-08 PF01035: DNA_binding_1" amino acids 196 to 275 (80 residues), 102.5 bits, see alignment E=1.7e-33 TIGR00589: methylated-DNA--[protein]-cysteine S-methyltransferase" amino acids 196 to 271 (76 residues), 95.9 bits, see alignment E=5.8e-32

Best Hits

KEGG orthology group: K10778, AraC family transcriptional regulator, regulatory protein of adaptative response / methylated-DNA-[protein]-cysteine methyltransferase [EC: 2.1.1.63] (inferred from 62% identity to fjo:Fjoh_1575)

Predicted SEED Role

"ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63)" in subsystem DNA repair, bacterial (EC 2.1.1.63)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.63

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G247 at UniProt or InterPro

Protein Sequence (282 amino acids)

>Echvi_3376 O-6-methylguanine DNA methyltransferase (Echinicola vietnamensis KMM 6221, DSM 17526)
MKDQEIITYKRVAEALNYLQNNFQRQPTLDEIADTVHLSPYHFQRIFTEWAGVSPKKFLQ
YISIGHAKKILSQPASTLSEASLETGLSGTGRLHDLFVNIEGMTPGEYKNGGENLGIDYQ
FFTTPFGRALVAATAKGICHLSFVEDDDHALQHLHSQFHKATFTKQPSPHHHEAMKIFHQ
DWHQMDTIKLHLKGSDFQLKVWECLLKIPQGNLSTYGHVAQHIHQPKAARAVGTAIGSNP
VAFLIPCHRVIQASGVIGGYMWGTLRKKAIIGWEAAAADRKG