Protein Info for Echvi_3239 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Topoisomerase IA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 693 PF01751: Toprim" amino acids 4 to 139 (136 residues), 41.8 bits, see alignment E=1.5e-14 PF01131: Topoisom_bac" amino acids 158 to 577 (420 residues), 392.7 bits, see alignment E=3.3e-121 PF13342: Toprim_Crpt" amino acids 630 to 688 (59 residues), 66.4 bits, see alignment 2.8e-22

Best Hits

KEGG orthology group: K03169, DNA topoisomerase III [EC: 5.99.1.2] (inferred from 82% identity to lby:Lbys_0184)

Predicted SEED Role

"DNA topoisomerase III (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G3Q2 at UniProt or InterPro

Protein Sequence (693 amino acids)

>Echvi_3239 Topoisomerase IA (Echinicola vietnamensis KMM 6221, DSM 17526)
MKTVIAEKPSVAREIASLLGATEKKEGHLTGNGYFVTWAFGHLVGLGMPEDYGITGFDKA
ALPILPHPFLLAVRKVKKDKGYTTDAGAIKQLKIIEQLFNRSDGIIVATDAGREGELIFR
YIYEYLKCIKPFQRLWISSLTEKAIKQGFDNLKDGKEFDGLYQAAQGRSRADWLVGINAT
QALSIAAGNGVYSLGRVQTPTLALICRRYLENKNFSVQKYWQIQLSHTKEFIEFKSLSKN
KWDNQKLAKDTLKSIERNGNTAAVTSVETKNVTEQPPLLFDLTGLQKEVNKKLNLSAEET
LKIAQSLYEKKFITYPRTGSKYIPEDMWTEIPNLIRALQDRDTCKQAVAKVKWGRFNKRI
VNDLRVTDHHGLLTTDTIPSALNAKENAVYDMIAFRLLEAISGACTKEATHITLQALHYD
FTTKGCKVLEPGWRSIKGNFTDDDAEPIEDLPDIKEGDVLKIKQAAVLEKKTKPPVLYTE
AGLLSAMETAGKDIKNEEERKALQNIGIGTPATRAAIIETLFTRNYIQREKKSLIPTEKG
LQVYELVKDRKIADVAMTAEWELALQKIETSEMNAIAFQKEMEAYTKSITGELLQTSIAK
DNSPELVCPKCKKQKLLIRDKIVKCPDEACNWVQFRIVCGVQIGIVDIESLVDNGKTSLI
KGMKSKAGKTFDAYIVLNDKAESSFEFEKRKKK