Protein Info for Echvi_3100 in Echinicola vietnamensis KMM 6221, DSM 17526
Annotation: mannonate dehydratase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 63% identical to UXUA_BACTN: Mannonate dehydratase (uxuA) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
KEGG orthology group: K01686, mannonate dehydratase [EC: 4.2.1.8] (inferred from 66% identity to hhy:Halhy_1062)MetaCyc: 59% identical to D-mannonate dehydratase (Escherichia coli K-12 substr. MG1655)
Mannonate dehydratase. [EC: 4.2.1.8]
Predicted SEED Role
"Mannonate dehydratase (EC 4.2.1.8)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 4.2.1.8)
MetaCyc Pathways
- superpathway of hexuronide and hexuronate degradation (9/10 steps found)
- D-fructuronate degradation (4/4 steps found)
- superpathway of β-D-glucuronosides degradation (6/7 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (14/31 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.8
Use Curated BLAST to search for 4.2.1.8
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0G1Y9 at UniProt or InterPro
Protein Sequence (382 amino acids)
>Echvi_3100 mannonate dehydratase (Echinicola vietnamensis KMM 6221, DSM 17526) MRWYGPQDPVTLADIRQAGATGIVSALHHLPNGVIWSREEIKKRKDTIEAAGLTWSVVES IPVHENIKTRSGNYQEYIANYKTSIGNLAAEGIHTVCYNFMPVLDWTRTDLAYELPNGAK ALRFELQALAAFDLFILQRPNAEKDYSEQVIKRAEAYFKEMTEDAEQLLTNNIIAGLPGA EEGYSLKAFQQVLDTYQHIDADKLATNLRLFLEEIIPTAEKNRVFMAIHPDDPPFPMFGL PRVMSTIDDVKRLVTETPSEYNGITFCTGSFGVRADNDLPQIIREHGDHIHFIHLRSTQR DAYGNFHEANHLEGDVPIVAVIKELIKVQSRRQKSLPMRPDHGHQMLDDLRKTTNPGYTG IGRLKGLAELRGLEMGLLHGLE