Protein Info for Echvi_3089 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Endopolygalacturonase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 477 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF12708: Pectate_lyase_3" amino acids 60 to 112 (53 residues), 33.9 bits, see alignment 2.8e-12 PF00295: Glyco_hydro_28" amino acids 223 to 457 (235 residues), 120.8 bits, see alignment E=6.8e-39

Best Hits

KEGG orthology group: None (inferred from 60% identity to rmr:Rmar_2391)

MetaCyc: 52% identical to exopolygalacturonase monomer (Thermotoga maritima)
Galacturan 1,4-alpha-galacturonidase. [EC: 3.2.1.67]

Predicted SEED Role

"Polygalacturonase (EC 3.2.1.15)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 3.2.1.15)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.15

Use Curated BLAST to search for 3.2.1.15 or 3.2.1.67

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G198 at UniProt or InterPro

Protein Sequence (477 amino acids)

>Echvi_3089 Endopolygalacturonase (Echinicola vietnamensis KMM 6221, DSM 17526)
MTHRYKFTSLLGMLFGLTLMVSCSKKEQSENTIVPSVWDQVPAILESIQKPSFPDQTFVI
SDFGAVGDGSTDASQAIKSAIQACAEAGGGKVVVPPGDYPTGPIYLESNVNLHLEKDARL
MFSTDPKDYLPLVYTRWEGVELMNYSPLVYAFEEENIAITGEGILDGQANETNWWPWKGK
TQYGYTEGDPQQEDADKRHALFQMAEDGVPVEERKFGEGFYLRPQFVQPYRCKNVLVEGV
KIVNSPMWILNPVLCENVTIEGVTVESHGPNSDGCDPESSKNVLIKDCYFNTGDDCIAIK
SGRNADGRRINVPSENIIIQNCKMADGHGGVVIGSEISGGVRNVFAENCEMNSPHLDRAL
RIKTSSMRGGIIEDIYLRNIDVGQIAQQVVRVNMFYEDSGAYVPTVRNIHVENMTVENGG
KVGVLLEGYENSPVENITLENVNIKNAEEAYTFSNVKGVRFKNVTINGKAVSYGQEE