Protein Info for Echvi_3057 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: citrate synthase I (hexameric type)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01798: citrate (Si)-synthase
  • amino acids 12 to 422 (411 residues), 584.2 bits, E=6e-180
• PF00285: Citrate_synt
  • amino acids 45 to 411 (367 residues), 468.8 bits, E=5.7e-145

Best Hits

Swiss-Prot: 58% identical to CISY1_MYCTO: Citrate synthase 1 (gltA2) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: K01647, citrate synthase [EC: 2.3.3.1] (inferred from 80% identity to mtt:Ftrac_0875)

MetaCyc: 58% identical to citrate synthase (Mycobacterium tuberculosis H37Rv)
Citrate (Si)-synthase. [EC: 2.3.3.1, 2.3.3.16, 2.3.3.3]

Predicted SEED Role

"Citrate synthase (si) (EC 2.3.3.1)" in subsystem Serine-glyoxylate cycle or TCA Cycle (EC 2.3.3.1)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle III (animals) (8/10 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (19/26 steps found)
• nitrogen remobilization from senescing leaves (6/8 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (10/14 steps found)
• glyoxylate cycle (4/6 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (6/9 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• superpathway of glyoxylate bypass and TCA (8/12 steps found)
• TCA cycle VII (acetate-producers) (5/9 steps found)
• mixed acid fermentation (10/16 steps found)
• methylaspartate cycle (11/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.1 or 2.3.3.16 or 2.3.3.3

Sequence Analysis Tools

See L0G373 at UniProt or InterPro

Protein Sequence (428 amino acids)

>Echvi_3057 citrate synthase I (hexameric type) (Echinicola vietnamensis KMM 6221, DSM 17526)
MSEIAKLSFNGTEYELPVTEGTENEKAIEIAKLRGQSGLITLDPGFKNTGSTKSAITFLD
GEKGILRYRGYNIEDLAEKSNFLEVSYLLIYGELPTQEQYDQFANEITYHTLVHEDIKKI
LDGFPSVAHPMGVLSSLICSLTAFYPTSLDPNRTDEEIKLSIVRLMAKLPTFAAWAYKNK
MGHPANYPDNSLDYCSNFMKMMFALPAEKYEVDPIIAKALDKLLILHADHEQNCSTSTVR
IVGSSQASIYASISAGINALWGPLHGGANQSVIEMLEAIKEDGGDTKKYLDKAKDKNDPF
RLMGFGHRVYKNFDPRAKIIKKAADDVLGKLGVNDPVLEIAKELEEAALNDQYFVDRKLY
PNVDFYSGIIYRALGIPTDMFTVMFALGRLPGWIAQWKEMRENNEPIGRPRQVYTGPNER
SYVSMGDR