Protein Info for Echvi_2994 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: DNA gyrase, B subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 652 TIGR01059: DNA gyrase, B subunit" amino acids 12 to 649 (638 residues), 962.8 bits, see alignment E=4.5e-294 PF02518: HATPase_c" amino acids 40 to 177 (138 residues), 62.4 bits, see alignment E=1e-20 PF00204: DNA_gyraseB" amino acids 232 to 401 (170 residues), 201.9 bits, see alignment E=1.1e-63 PF01751: Toprim" amino acids 430 to 544 (115 residues), 64.8 bits, see alignment E=1.4e-21 PF00986: DNA_gyraseB_C" amino acids 582 to 642 (61 residues), 107.7 bits, see alignment 5.3e-35

Best Hits

Swiss-Prot: 58% identical to GYRB_BACSU: DNA gyrase subunit B (gyrB) from Bacillus subtilis (strain 168)

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 76% identity to mtt:Ftrac_0291)

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G104 at UniProt or InterPro

Protein Sequence (652 amino acids)

>Echvi_2994 DNA gyrase, B subunit (Echinicola vietnamensis KMM 6221, DSM 17526)
MSKEQASNLGEYSAGNIQILEGLEAVRKRPAMYIGDVGVKGLHHLIWEVVDNSIDEALAG
HCDTIHVTVLEDNSVQVEDNGRGIPTDMHEKEKRSALEVVMTVLHAGGKFDKDTYKVSGG
LHGVGVSCVNALSTHLKATVYRNGKVFEQEYSRGVPQTGVTEIGETEKRGTIIHFVPDGD
IFTVTEYKYETIANRLREMAFLNAGVRIFFKDMRELDEEGNPKSDEFFSEGGLIEFVEYL
DETREKIIQEPIYIESEKNAVPVQVAMSYNSSFSENVVSYVNTINTYEGGTHVSGFRRAL
TRTLKGYADKSGMLDKVKFDVSGDDFREGLTAVISVKVAEPQFEGQTKTKLGNSEVVSAV
DSAVSEVLQYWLEEHPKEAKIIVGKVVLAAQARHAARKAREMVQRKNVLGGGGLPGKLAD
CANTNPEVCELYLVEGDSAGGSAKQGRDRDFQAILPLKGKILNVEKAHEHKIYDNDEIKN
ILTALGVKFGTAEDEKELDLTNLRYHKIVIMTDADIDGSHIRTLILTLFFRYMRSLIENG
YVYIALPPLYLLKKGKDERYCWSEDERIKLTKEMAGDGKTDNVGIQRYKGLGEMNPEQLW
TTTMDPESRTLKQVTIESAAEADHLFSMLMGDEVAPRREFIEKNAKYAKIDT