Protein Info for Echvi_2850 in Echinicola vietnamensis KMM 6221, DSM 17526

Updated annotation (from data): Alpha-amylase (EC 3.2.1.1)
Rationale: Specifically important for utilizing a-Cyclodextrin. Automated validation from mutant phenotype: the predicted function (3.2.1.1) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: Glycosidases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 519 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF00128: Alpha-amylase" amino acids 43 to 444 (402 residues), 325.6 bits, see alignment E=6.6e-101 PF23915: SusG_C" amino acids 459 to 516 (58 residues), 57.7 bits, see alignment 1.2e-19

Best Hits

Predicted SEED Role

"Alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.1

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FYW1 at UniProt or InterPro

Protein Sequence (519 amino acids)

>Echvi_2850 Alpha-amylase (EC 3.2.1.1) (Echinicola vietnamensis KMM 6221, DSM 17526)
MRNLLRSFTYCTLVLLTASCMQDANEELEVKNYWPEAGITYEIFIQSFYDTDGDGIGDIN
GVTKKLDHVQELGANAIWFMPLMPSPSYHKYDVTDYKAIHPDYGTMDDFKQMLDEAHKRD
IKVVIDMIINHTSDEHPWFQEAKKGRDNPYRDYYVWAQYDTIQDYLDKKVVTLDSDNIRQ
WHDPGQGDDYYYGFFTGDMPDLNFDNPKVREEIYEIGRYWLAEVGVDGFRLDAAKHIYPD
DRAADSHEFWEEFRAEMEKVKPDVYLVGEVYDMKEVVAPYLTGLRALFNFDFHYTLLEAY
KKEDGMLLAKKQHDILAFYNGITDDFIDATISSNHDQPRLLNELGKSKDKLKQAIAILMT
MPGAPYIYYGEEIGMLGKKPDPNIREPFLWDVAEQDEGRTKWITPAFSTDNTVTPLAIQK
EDADSYFNHYKRVIQLRNTHPALAIGSLELPAEKYPKAVMAYQRKTGEQELYVFHNLGKK
SVEIPLPQGFDREVYHLKGAKVNGDKIKLPAFSSIVLGK