Protein Info for Echvi_2835 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: RND family efflux transporter, MFP subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 transmembrane" amino acids 9 to 32 (24 residues), see Phobius details PF16576: HlyD_D23" amino acids 53 to 292 (240 residues), 54.3 bits, see alignment E=2.3e-18 PF13533: Biotin_lipoyl_2" amino acids 67 to 113 (47 residues), 28.3 bits, see alignment 2.4e-10 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 125 to 407 (283 residues), 96.6 bits, see alignment E=6.8e-32 PF13437: HlyD_3" amino acids 205 to 310 (106 residues), 36.2 bits, see alignment E=1.7e-12

Best Hits

KEGG orthology group: K02005, HlyD family secretion protein (inferred from 56% identity to mtt:Ftrac_1033)

Predicted SEED Role

"Macrolide-specific efflux protein MacA" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FYU7 at UniProt or InterPro

Protein Sequence (428 amino acids)

>Echvi_2835 RND family efflux transporter, MFP subunit (Echinicola vietnamensis KMM 6221, DSM 17526)
MAKKKSNKLIYILGGVAGVLILFIIIGRALGWVGGAAETEVETTKASKKTIVEKVSASGV
IEPETEVKLSPDVAGEIIELNISEGDSVKQNDLLVKIRPDNFISALDRTRANLNQQKANL
AQSQAALKRSEALFDRAKLQYERNKSLYESNVISDSEFEQATADYVSAENDLKAAQQSVE
AAEYVVKSSQASVEEANENLRLTNVFAPTDGIVSKLLVEKGERVVGTQQMAGTEMLRIAD
LSVMEVVVDVNENDIVRISLGDTTIIEVDAYSHTGEKFTGVVTSIANSANEKATQDAVTE
FEVEIRILNESYEKLITKENRYPFRPGMTASVEIITEKKADVLSVPLAAVTTRDDIRVDS
TNAESDLQEIIFMSEGNKAHLTRVKTGISDFENIEILEGIKEGEELVSGPYFVVSKQLED
GDLIKKTN