Protein Info for Echvi_2777 in Echinicola vietnamensis KMM 6221, DSM 17526

Updated annotation (from data): fused D-3-phosphoglycerate dehydrogenase / phosphoserine phosphatase (EC 1.1.1.95; EC 3.1.3.3)
Rationale: Important for fitness in defined media, unless serine is added as a nitrogen source. The N-terminal part is distantly related to the phosphoserine phosphatase from Arabidopsis, and no other candidate was found for this step. The C-terminal part is 52% identical to E. coli phosphoglycerate dehydrogenase.
Original annotation: Haloacid Dehalogenase superfamily, subfamily IB, phosphoserine phosphatase-like

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 630 TIGR01488: HAD phosphoserine phosphatase-like hydrolase, family IB" amino acids 7 to 180 (174 residues), 45.6 bits, see alignment E=3.8e-16 PF12710: HAD" amino acids 8 to 180 (173 residues), 32.9 bits, see alignment E=1.8e-11 PF00389: 2-Hacid_dh" amino acids 235 to 547 (313 residues), 119.1 bits, see alignment E=2.2e-38 PF02826: 2-Hacid_dh_C" amino acids 341 to 515 (175 residues), 168.6 bits, see alignment E=1.9e-53 PF03446: NAD_binding_2" amino acids 374 to 475 (102 residues), 24.1 bits, see alignment E=6.7e-09

Best Hits

KEGG orthology group: K00058, D-3-phosphoglycerate dehydrogenase [EC: 1.1.1.95] (inferred from 68% identity to zpr:ZPR_2876)

Predicted SEED Role

"D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 1.1.1.95)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.95

Use Curated BLAST to search for 1.1.1.95 or 3.1.3.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G228 at UniProt or InterPro

Protein Sequence (630 amino acids)

>Echvi_2777 fused D-3-phosphoglycerate dehydrogenase / phosphoserine phosphatase (EC 1.1.1.95; EC 3.1.3.3) (Echinicola vietnamensis KMM 6221, DSM 17526)
MTTDKKFIIDFDSTFTQVEALDILGEISLRNDPKRDEKLQAIKDITDLGMEGKLNLRESL
ERRIEILQANKSQIAELIDALKQKVSKSFQRNREFFQENAENIYILSNGFKDFITPVVAA
YGLKEENVFANDFIYDEAGNIIDLNKENLLSNNNGKPATIKSLKLEGDVYVIGDGYTDYE
IKASGLANKFYAFTENINRPKVSSKADHIAPSLDEILYVNKMNKKFSYPKSRINVLLLEN
VHPIGVEIMKQEGYNVEVVSSAMSEEELCEKIKNVSIIGIRSKTQITKKVLENANRLMAV
GAFCIGTNQIDLETCQEKGIAVFNAPFSNTRSVVELAISEIIFLMRNLHDKTLKMHQGIW
NKSASGSFEVRGKKLGIIGYGNIGAQLSVLAENMGMNVFYYDIVERLALGNATKIDSLDE
LLETCDIISLHVDGRTENKNILNKEKIFKMKKGAILVNLSRGHVVDVPALRDALESGHLA
GAAVDVFPTEPKNNDEPFESELIGCPNTILTPHIGGSTLEAQENIAQFVPGKIIEYINSG
NTFNSVNFPNIQLPFLKDAHRLIHIHQNAPGVLAKINQVLASYKINIVGQYLKTNEKIGY
VITDIDKRYSNDVIDALKEIEGTIRFRILY