Protein Info for Echvi_2559 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08267: Meth_synt_1
  • amino acids 3 to 317 (315 residues), 403 bits, E=1.1e-124
  • amino acids 560 to 699 (140 residues), 34.8 bits, E=1.4e-12
• TIGR01371: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
  • amino acids 6 to 766 (761 residues), 1118.1 bits, E=0
• PF01717: Meth_synt_2
  • amino acids 440 to 762 (323 residues), 523.4 bits, E=2.5e-161

Best Hits

Swiss-Prot: 64% identical to METE_FLAJ1: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) from Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101)

KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 64% identity to fjo:Fjoh_1519)

MetaCyc: 52% identical to cobalamin-independent methionine synthase MS2 (Arabidopsis thaliana col)
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase. [EC: 2.1.1.14]

Predicted SEED Role

"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)

MetaCyc Pathways

• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (15/18 steps found)
• aspartate superpathway (20/25 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• S-adenosyl-L-methionine salvage II (3/3 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• S-adenosyl-L-methionine salvage I (2/4 steps found)
• superpathway of seleno-compound metabolism (8/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.14

Sequence Analysis Tools

See L0G1B3 at UniProt or InterPro

Protein Sequence (775 amino acids)

>Echvi_2559 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase (Echinicola vietnamensis KMM 6221, DSM 17526)
MLTQNLGYPRIGSKRQLKKACEAYWAGKIPLNDLQKAAAEIRETNWKLQQQAGLDLIPSN
DFSFYDQVLDMAFTVGAIPGRYHEILTELKKPELDLYFAMARGYQKDGLDIIAMEMTKWF
DTNYHYIVPEFTKDQQFKLFSTKVLDEFKEAKRLGIHTKPVLLGPVSFLLLGKEKEAGFH
RIDLLKALLPVYIDLLKKLESLHADWVQLDEPFLVMDLKDEEKAAVRWAYQEIKKAVPGI
KTLLTTYFGALDDNLTLAAGLPICGLHVDLVRNPEQLEAVLEKVPANMSLSLGLVDGRNI
WKNDYQASLELINKARAAKQDERLLIGPSCSLLHSPCDLEEESNEHTLSAELKDWLAFAR
QKTTEITALKVIVEDAGSAKSKALLEENQASIAKKRTSERIHKPAVRKAVEGLTPNHSQR
KSAFASRKTIQAEKLYLPDFPTTTIGSFPQTKEVRQWRSQLKKEVLTENQYVDNIKAATQ
EAIRWQEEVGLDVLVHGEFERNDMVEYFGEKLEGFAFTQYGWVQSYGSRCVKPPIIYGDV
SRPKPMTLFWTTFAQSLTDKPVKGMLTGPVTILQWSFVRNDQSRKATCQQIALAIREEVK
DLEKSGIQIIQIDEPALREGLPLRHAEWNDYLQWAVECFRISASAVEDSTQIHTHMCYSE
FNDIIEHIAAMDADVITIECSRSQMELLDAFAQFNYPNDIGPGVYDIHSPRVPASEEMVT
LMQKAQAVVPYRQLWVNPDCGLKTRGWEETKEALGRMVEAARKLRAAITSNVTVS