Protein Info for Echvi_2517 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: phosphoribosyl-ATP pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 201 PF01502: PRA-CH" amino acids 29 to 101 (73 residues), 117.5 bits, see alignment E=1.9e-38 TIGR03188: phosphoribosyl-ATP diphosphatase" amino acids 113 to 195 (83 residues), 101.2 bits, see alignment E=1.5e-33 PF01503: PRA-PH" amino acids 114 to 199 (86 residues), 59.8 bits, see alignment E=2.6e-20

Best Hits

Swiss-Prot: 61% identical to HIS2_BACTN: Histidine biosynthesis bifunctional protein HisIE (hisI) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K11755, phosphoribosyl-ATP pyrophosphohydrolase / phosphoribosyl-AMP cyclohydrolase [EC: 3.5.4.19 3.6.1.31] (inferred from 71% identity to chu:CHU_2337)

MetaCyc: 53% identical to putative bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase (Escherichia coli K-12 substr. MG1655)
Phosphoribosyl-ATP diphosphatase. [EC: 3.6.1.31]; Phosphoribosyl-AMP cyclohydrolase. [EC: 3.6.1.31, 3.5.4.19]

Predicted SEED Role

"Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) / Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)" in subsystem Histidine Biosynthesis (EC 3.5.4.19, EC 3.6.1.31)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.19 or 3.6.1.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G0D7 at UniProt or InterPro

Protein Sequence (201 amino acids)

>Echvi_2517 phosphoribosyl-ATP pyrophosphohydrolase (Echinicola vietnamensis KMM 6221, DSM 17526)
MENLKIDFDKVNGLVPAIIQDATTNAVLMLGYMNEEALQKTQESGKVTFFSRTKQRLWTK
GETSGNFMHVQSIKVDCDNDTLLIKADPVGPVCHTGADTCFDEKNTSKTAFIDQLRSIIK
DRKNNPTDKSYTASLFAKGINKVAQKVGEEAVEIVIEAKDDHKDLFMGEAADLLFHYLVL
LEAKGYELDEVMDVLIQRHKG