Protein Info for Echvi_2479 in Echinicola vietnamensis KMM 6221, DSM 17526
Annotation: pyrroline-5-carboxylate reductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00286, pyrroline-5-carboxylate reductase [EC: 1.5.1.2] (inferred from 53% identity to cpi:Cpin_0657)Predicted SEED Role
"Pyrroline-5-carboxylate reductase (EC 1.5.1.2)" in subsystem Proline Synthesis (EC 1.5.1.2)
MetaCyc Pathways
- L-arginine degradation XIII (reductive Stickland reaction) (5/5 steps found)
- L-arginine degradation VI (arginase 2 pathway) (4/4 steps found)
- L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
- L-proline biosynthesis III (from L-ornithine) (3/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.5.1.2
Use Curated BLAST to search for 1.5.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0G132 at UniProt or InterPro
Protein Sequence (265 amino acids)
>Echvi_2479 pyrroline-5-carboxylate reductase (Echinicola vietnamensis KMM 6221, DSM 17526) MKNLKIAIIGCGNLGLSIVNGLLETEGFEAKNLMVTKRHPENLSHLQSLGVTVTADNKSA AKAADLVILGVKPYNIPHILKEIAPVVSPDKQTIISLATGVTLEEMYQHLPSTTALYRAM PNIAADIQESITCICGQNSTPENEDIIKALFNSIGISIIIEEHLMEAATVLGACGIAYVL RFMRAMTQGGIQIGFDAKTANTIVNQTVKGAAELLIKKGIHPEAAIDKVTTPKGCTIVGL NEMEHHGFSAAMVKGVLASYEKIEK