Protein Info for Echvi_2467 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: arginyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 596 TIGR00456: arginine--tRNA ligase" amino acids 3 to 596 (594 residues), 420.9 bits, see alignment E=3.6e-130 PF00750: tRNA-synt_1d" amino acids 97 to 451 (355 residues), 151.4 bits, see alignment E=3.4e-48 PF05746: DALR_1" amino acids 476 to 596 (121 residues), 103.5 bits, see alignment E=8e-34

Best Hits

Swiss-Prot: 60% identical to SYR_AMOA5: Arginine--tRNA ligase (argS) from Amoebophilus asiaticus (strain 5a2)

KEGG orthology group: K01887, arginyl-tRNA synthetase [EC: 6.1.1.19] (inferred from 62% identity to dfe:Dfer_2678)

Predicted SEED Role

"Arginyl-tRNA synthetase (EC 6.1.1.19)" (EC 6.1.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FXS2 at UniProt or InterPro

Protein Sequence (596 amino acids)

>Echvi_2467 arginyl-tRNA synthetase (Echinicola vietnamensis KMM 6221, DSM 17526)
MTIQEQILKAISEGFSKAFDHQVTPESLSLQPTRKEFEGNYTFVLFPFLKYTKMKPEDSG
DKIGQYLVENCEAVSAFNVVKGFLNISVNEQSWMAVFQTLYQNEQLGQLPPKNQKVMVEF
SSPNTNKPLHLGHLRNNFLGFSVSEILAANGYEVIKANLVNDRGIHICKSMVAYLHFGNG
ETPTSGGFKGDHLAGKYYVLFDKAYKKQINELIEKGLSEEDAKKQAPLMLEAQEMLRKWE
AGDEETVTLWKTMNSWVYEGFDATYKKMGVSFDKFYYESDTYLLGKNIVEEGLEKGVFFK
KDNNSVWADLTEEGLDEKLVLRGDGTSVYITQDMGTADLKYEDFKINKSVYVVGNEQDYH
FDVLFKIMRKLNRPYGEGLYHLSYGMVDLPTGKMKSREGTVVDADDLIQEMVDTAESHTK
ELGKIEGFSDEQAKELYEMLGLGALKYFLLKVDPKKRMLFNPQESIEFQGNTGPFIQYSH
ARIASILRKAKQIGLDYSASGFNGLQQVENAEKELIILLNDYEKKIAQAGEELSPSVIAQ
YMFDLAKEYNRFYAELPIFSEADEKIRSFRVALSAQVAKTLQSGMKLLGIKVPEKM