Protein Info for Echvi_2312 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: NAD+ synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00795: CN_hydrolase
  • amino acids 11 to 245 (235 residues), 67.3 bits, E=1.4e-22
• TIGR00552: NAD+ synthetase
  • amino acids 285 to 583 (299 residues), 84.5 bits, E=3.5e-28
• PF02540: NAD_synthase
  • amino acids 285 to 576 (292 residues), 98.9 bits, E=2.6e-32

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

• aspartate superpathway (20/25 steps found)
• NAD de novo biosynthesis II (from tryptophan) (8/9 steps found)
• NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde (4/4 steps found)
• NAD salvage pathway I (PNC VI cycle) (6/7 steps found)
• superpathway of NAD biosynthesis in eukaryotes (11/14 steps found)
• NAD salvage pathway II (PNC IV cycle) (4/5 steps found)
• NAD salvage pathway V (PNC V cycle) (4/5 steps found)
• NAD de novo biosynthesis I (4/6 steps found)
• NAD de novo biosynthesis III (4/6 steps found)
• NAD de novo biosynthesis IV (anaerobic) (4/6 steps found)
• NAD salvage (plants) (6/11 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.5

Sequence Analysis Tools

See L0G157 at UniProt or InterPro

Protein Sequence (617 amino acids)

>Echvi_2312 NAD+ synthetase (Echinicola vietnamensis KMM 6221, DSM 17526)
MSILKIGGATVNQTPLDWEGNLKNITDAIQEAKSQGIELLCFPELAITGYGSEDLFLSRW
YPDKSLAQLQKLLPYAKDITICIGLPVRIGETLYNCTAVLENQKVRGIMAKQFLAIDGVH
YEFRWFSPWQAGKMTTLDFFGENIPFGDMVFDKKGITYGFEICEDAWRGDLRPGYRLKDR
HVDLIFNPSASHFAMGKSAHREDLVTESSIALDATYFYVNLLGNEAGRMIFDGEILAAQH
GKLLLKNPLLSFKPYQVRAFDFDKDAIPHEEAVSEHDKNEEFTAAVCLALFDYMRKSRST
GFVLSLSGGADSSSIATLVAEMVHRGIHELGLDAFLERAHISQLSSTENSTKAVVQKILT
TAYQGSDNSSADTLESARCLAESLGATFYDWKISTEVSSYTKKIETAIGRQLTWDQDDIT
LQNIQARVRAPAIWMLANLNNALLLATSNRSEGDVGYATMDGDTSGSISPIAAVDKDFIL
QWLQWAEKHLGYSGLHKVNALQPTAELRPQEQHQTDEQDLMPYPVIVEIERLAIRDRRKP
ADIYLILKQELDISPVQLKKYIQKFFRLWSRNQWKRERLAPSFHLDDFNVDPKTWCRFPI
LSGGFTEELRELDRLDE