Protein Info for Echvi_2275 in Echinicola vietnamensis KMM 6221, DSM 17526

Updated annotation (from data): translesion DNA polymerase (imuC-like)
Rationale: Specifically important in stress Cisplatin. Distantly related (less than 30% identity) to imuC (also known as dnaE2), which is a translesion DNA polymerase that is involved in DNA repair in Pseudomonas and Mycobacteria (see PMC3090020).
Original annotation: DNA polymerase III, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1002 PF02811: PHP" amino acids 5 to 85 (81 residues), 39.1 bits, see alignment E=2.1e-13 PF07733: DNA_pol3_alpha" amino acids 246 to 488 (243 residues), 202.3 bits, see alignment E=2.3e-63 PF17657: DNA_pol3_finger" amino acids 494 to 661 (168 residues), 114.2 bits, see alignment E=8.3e-37 PF14579: HHH_6" amino acids 736 to 819 (84 residues), 52 bits, see alignment E=1.4e-17

Best Hits

KEGG orthology group: K02337, DNA polymerase III subunit alpha [EC: 2.7.7.7] (inferred from 53% identity to rbi:RB2501_04555)

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FZU0 at UniProt or InterPro

Protein Sequence (1002 amino acids)

>Echvi_2275 translesion DNA polymerase (imuC-like) (Echinicola vietnamensis KMM 6221, DSM 17526)
MYVNCHSYFSFRYGTMPVQEMVDQAKKLGVKAMALTDINCTAAVYPFLKATKAANIHGVI
GIDFRNGIDQQYIGLALNHHGFFEMNRHLSDHKGKKRPFPQKAPQWQNTAVIYPFPQPYF
KLGPREYLGIPADQLKKALRSPWIAEKDRWILLQSVTFHSQQSFNIHRLLRCIELNILLS
KLPTSEQGNLSDRFYSTLELAERCAPHYWLLQQTHALLSRCRFEQDYQPVANKKSVYGSE
QEDLAQLTALTYQGAKNRYGTPLSERQETRILKELDIIRQKDFVSYFLINYEIVKFARER
GFYYVGRGSGANSIVAYCLNITDVDPIELDLYFERFINLYRSTPPDFDIDFSWRDRDEVI
QHIFHKHNTEDHEHVCLLATHNTFQINSSIRELGKVFGLPKTDIEALINHVVKPGNYIPD
RIGALVLKYSQLIHGMPSHLSIHAGGILISQQPIHYYTATDLPPKGFPISHFDMVTAEEI
GFAKFDILSQRGLGHIRDSLEIIEKNQGKKVDIHRIKDFKHDEKIKQHLRKGHTIGAFYV
ESPAMRMLLAKLEVDEYLGLVAASSIIRPGVARSGMMREYILRHRKPEMRKSRAHPILHD
LMPETYGIMVYQEDVIKVAHYFAGLSFDEADVLRRGMSGKFRSRAAFLKVKESFFKKSME
KGHAPKITSEIWFQVESFAGYSFAKGHSASFAVESYQSLYLKAHFPLEFMVGVINNFGGF
YKTEFYIRELQGYHADVQLPHLNESDYLTVIKGTTVYLGFIHLKYLQKDITNTILQNRET
EGPFTGLRDFINRIAISLEQLLILIRIDAFRFTAKRKQELLWEAHFLTNKQKNKPSTNHL
FRQINLRELSIPHLETNDPRQDILDQLEIMEFSIADPFLLLKAPPHDTLKAQDIPSYAGK
EIRLMGYLVTVKYTRTVKGDIMNFGTFLDRDGQWIDTVHFPPVIRQYPLTGRGIYLLEGK
VSEEFNFYTIEISSCKKLAYWNAADDHPNPVTNQSPKTSIDN