Protein Info for Echvi_2061 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Isopropylmalate/homocitrate/citramalate synthases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 504 PF00682: HMGL-like" amino acids 1 to 265 (265 residues), 176.4 bits, see alignment E=8.5e-56 PF08502: LeuA_dimer" amino acids 361 to 494 (134 residues), 71.1 bits, see alignment E=8.5e-24

Best Hits

KEGG orthology group: K09011, D-citramalate synthase [EC: 2.3.1.182] (inferred from 66% identity to fjo:Fjoh_1305)

MetaCyc: 53% identical to (R)-citramalate synthase subunit (Leptospira interrogans serovar Lai str. 56601)
RXN-7743 [EC: 2.3.3.21]

Predicted SEED Role

"(R)-citramalate synthase (EC 2.3.1.182)" in subsystem Branched-Chain Amino Acid Biosynthesis (EC 2.3.1.182)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.182 or 2.3.3.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FZ33 at UniProt or InterPro

Protein Sequence (504 amino acids)

>Echvi_2061 Isopropylmalate/homocitrate/citramalate synthases (Echinicola vietnamensis KMM 6221, DSM 17526)
MDTTLRDGEQTSGVSFLPSEKLQIAKLLLEELRVDRIEVASARVSEGELEGVKKITHWAA
EKGYLDCVEVLGFVDTPASVDWLTEAGAKVLNLLTKGSLNHLTHQLKKTPVEHFAAIEKC
IHYANEKGISVNVYLEDWSSGMRHSRDYTLELIAFLADQNVKRVMLPDTLGLLKPAEVAE
YVGLVSEQFPEVHFDFHAHNDYDLSVANVMEAINHGISGIHTTVNGLGERAGNAPLESVV
ATLSDFTTVKLNVQENKIYRISKLVEQFSGLHIPSNKPVVGENVFTQTAGIHADGDNKKN
LYFNDLLPERFGRTRKYALGKTSGKANILKNLLELGIKLEPEELSKVTQKIIELGDRKER
VTTEDLPYIISDVLQNNSIKKDISIEGYHMTHSKGLKPTVQLKLKFKDQFYEAHASGNGQ
FDSFMLALQKIYKSLNKKLPKLTDFSVSIPPGGKTDAFVETVITWDYGRIIKTKGLDSDQ
TVAAMMATEKMLNIIEQIDSGNRA